Abstract
Nuclear envelope (NE) formation during cell division in multicellular organisms is a central yet poorly understood biological process. We report that the conserved nucleoporin Nup155 has an essential function in NE formation in Caenorhabditis elegans embryos and in Xenopus laevis egg extracts. In vivo depletion of Nup155 led to failure of nuclear lamina formation and defects in chromosome segregation at anaphase. Nup155 depletion inhibited accumulation of nucleoporins at the nuclear periphery, including those recruited to chromatin early in NE formation. Electron microscopy analysis revealed that Nup155 is also required for the formation of a continuous nuclear membrane in vivo and in vitro. Time-course experiments indicated that Nup155 is recruited to chromatin at the time of NE sealing, suggesting that nuclear pore complex assembly has to progress to a relatively late stage before NE membrane assembly occurs.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Caenorhabditis elegans / genetics
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Caenorhabditis elegans / metabolism*
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Caenorhabditis elegans / ultrastructure
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Caenorhabditis elegans Proteins / analysis
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Caenorhabditis elegans Proteins / genetics
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Caenorhabditis elegans Proteins / metabolism*
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Cell Nucleus / chemistry
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Cell Nucleus / ultrastructure
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Chromatin / chemistry
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Chromatin / metabolism
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Chromosome Segregation / genetics
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Embryo, Nonmammalian / metabolism
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Nuclear Envelope / genetics
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Nuclear Envelope / metabolism
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Nuclear Envelope / ultrastructure*
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Nuclear Lamina / genetics
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Nuclear Lamina / metabolism
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Nuclear Pore / metabolism
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Nuclear Pore Complex Proteins / analysis
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Nuclear Pore Complex Proteins / genetics
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Nuclear Pore Complex Proteins / metabolism*
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RNA Interference
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Xenopus Proteins / analysis
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Xenopus Proteins / genetics
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Xenopus Proteins / metabolism*
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Xenopus laevis / genetics
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Xenopus laevis / metabolism*
Substances
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Caenorhabditis elegans Proteins
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Chromatin
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NUP155 protein, Xenopus
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Nuclear Pore Complex Proteins
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Xenopus Proteins
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npp-8 protein, C elegans