Abstract
Recombination enhancer (RE) is essential for regulating donor preference during yeast mating type switching. In this study, by using minichromosome affinity purification (MAP) and mass spectrometry, we found that yeast Ku80p is associated with RE in MATa cells. Chromatin immunoprecipitation assays confirmed its occupancy in vivo. Deletion of YKU80 results in altered chromatin structure in the RE region and more importantly causes a dramatic decrease of HML usage in MATa cells. We also detect directional movement of yKu80p from the RE towards HML during switching. These results indicate a novel function of yeast Ku80p in regulating mating type switching.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Binding Sites
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Chromatin / metabolism
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Chromatin Immunoprecipitation
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Chromosomes / metabolism
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Chromosomes / ultrastructure
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DNA Repair
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DNA-Binding Proteins / metabolism
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DNA-Binding Proteins / physiology*
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Electrophoresis, Polyacrylamide Gel
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Epitopes / chemistry
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Gene Deletion
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Genes, Fungal*
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Mass Spectrometry
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Micrococcal Nuclease / metabolism
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Models, Genetic
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Nucleosomes / metabolism
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Protein Binding
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Protein Structure, Tertiary
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Recombination, Genetic*
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Saccharomyces cerevisiae Proteins / metabolism
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Saccharomyces cerevisiae Proteins / physiology*
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Time Factors
Substances
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Chromatin
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DNA-Binding Proteins
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Epitopes
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Nucleosomes
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Saccharomyces cerevisiae Proteins
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YKU80 protein, S cerevisiae
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high affinity DNA-binding factor, S cerevisiae
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Micrococcal Nuclease