Abstract
Fission yeast mutants defective in DNA replication have widely varying morphological phenotypes. We designed a screen for temperature-sensitive mutants defective in the process of replication regardless of morphology by isolating strains unable to rereplicate their DNA in the absence of cyclin B (Cdc13). Of the 42 rereplication-defective mutants analyzed, we were able to clone complementing plasmids for 10. This screen identified new alleles of the APC subunit cut9(+), the initiation/checkpoint factor rad4(+)/cut5(+), and the first mutant allele of psf2(+), a subunit of the novel GINS replication complex. Other genes identified are likely to play general roles in gene expression and protein localization.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Alleles
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Apc6 Subunit, Anaphase-Promoting Complex-Cyclosome
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Cell Cycle*
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Cyclin B / deficiency
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DNA Replication*
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DNA, Fungal / genetics*
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DNA-Binding Proteins / chemistry
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DNA-Binding Proteins / genetics
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Genetic Complementation Test
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Mutation*
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Nuclear Proteins / chemistry
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Nuclear Proteins / genetics
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Plasmids
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Schizosaccharomyces / genetics*
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Schizosaccharomyces pombe Proteins* / chemistry
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Schizosaccharomyces pombe Proteins* / genetics
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Temperature
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Transglutaminases / chemistry
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Transglutaminases / genetics
Substances
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Apc6 Subunit, Anaphase-Promoting Complex-Cyclosome
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CUT9 protein, S pombe
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Cyclin B
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DNA, Fungal
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DNA-Binding Proteins
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Nuclear Proteins
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RAD4 protein, S pombe
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Schizosaccharomyces pombe Proteins
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Transglutaminases