Abstract
We here demonstrated that the Soh1/MED31 protein is a stable component of Mediator complex isolated from Schizosaccharomyces pombe and Saccharomyces cerevisiae. Bioinformatic analysis traces the Soh1/MED31 family of Mediator subunits to the point of major eukaryotic divergence, before the appearance of the canonical heptapeptide repeat structure of the RNA polymerase II C-terminal domain.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Motifs
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Amino Acid Sequence
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Animals
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Computational Biology
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Conserved Sequence
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Electrophoresis, Polyacrylamide Gel
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Escherichia coli / metabolism
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Evolution, Molecular
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Glutathione Transferase / metabolism
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Humans
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Immunoblotting
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Macromolecular Substances / chemistry
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Mediator Complex
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Molecular Sequence Data
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Plasmids / metabolism
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Protein Structure, Tertiary
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Protein Subunits / chemistry
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Protein Subunits / metabolism
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RNA Polymerase II / chemistry
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae / physiology*
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Saccharomyces cerevisiae Proteins / physiology*
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Schizosaccharomyces / genetics
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Schizosaccharomyces / physiology*
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Sequence Homology, Amino Acid
Substances
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Macromolecular Substances
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Mediator Complex
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Protein Subunits
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SOH1 protein, S cerevisiae
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Saccharomyces cerevisiae Proteins
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Glutathione Transferase
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RNA Polymerase II