GAR1 is an essential small nucleolar RNP protein required for pre-rRNA processing in yeast

EMBO J. 1992 Feb;11(2):673-82. doi: 10.1002/j.1460-2075.1992.tb05099.x.

Abstract

Among the few proteins of the eukaryotic nucleolus that have been characterized, four proteins, nucleolin, fibrillarin, SSB1 and NSR1, possess a common structural motif, the GAR domain, which is rich in glycine and arginine residues. In order to examine whether the presence of this domain is characteristic of a family of nucleolar proteins, we investigated whether other yeast genes encode proteins containing GAR domains. We report here the sequence and the characterization of a new yeast gene, GAR1, which encodes a protein of 205 residues containing two GAR domains. GAR1 is a non-ribosomal protein, localized in the yeast nucleolus, which is essential for cell growth. Immunoprecipitation with anti-GAR1 antibodies shows that GAR1 is associated with a subset of snoRNAs, including snR10 and snR30. Depletion of GAR1 by expression under the control of a regulated GAL promoter, impairs processing of the 35S primary transcript of pre-rRNA and prevents synthesis of 18S rRNA. GAR1 is thus the fifth member of a family of nucleolar proteins containing GAR domains, and is involved in rRNA metabolism.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Blotting, Southern
  • Cloning, Molecular
  • DNA, Fungal / genetics
  • DNA, Fungal / isolation & purification
  • Escherichia coli / genetics
  • Fungal Proteins / genetics*
  • Genes, Fungal*
  • Molecular Sequence Data
  • Multigene Family
  • Nuclear Proteins / genetics*
  • RNA Precursors / genetics*
  • RNA Processing, Post-Transcriptional*
  • Recombinant Fusion Proteins / metabolism
  • Restriction Mapping
  • Ribonucleoproteins / genetics*
  • Ribonucleoproteins / metabolism*
  • Ribonucleoproteins, Small Nuclear
  • Ribonucleoproteins, Small Nucleolar*
  • Ribosomes / metabolism
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae Proteins*
  • beta-Galactosidase / genetics
  • beta-Galactosidase / metabolism

Substances

  • DNA, Fungal
  • Fungal Proteins
  • Nuclear Proteins
  • RNA Precursors
  • Recombinant Fusion Proteins
  • Ribonucleoproteins
  • Ribonucleoproteins, Small Nuclear
  • Ribonucleoproteins, Small Nucleolar
  • Saccharomyces cerevisiae Proteins
  • GAR1 protein, S cerevisiae
  • beta-Galactosidase

Associated data

  • GENBANK/D13200
  • GENBANK/D13201
  • GENBANK/D13202
  • GENBANK/D13203
  • GENBANK/L07339
  • GENBANK/M94783
  • GENBANK/M94784
  • GENBANK/M94785
  • GENBANK/X63350
  • GENBANK/X63617