The Iml3 protein of the budding yeast is required for the prevention of precocious sister chromatid separation in meiosis I and for sister chromatid disjunction in meiosis II

Santanu Kumar Ghosh; Soumitra Sau; Sudeshna Lahiri; Anuradha Lohia; Pratima Sinha

doi:10.1007/s00294-004-0516-6

The Iml3 protein of the budding yeast is required for the prevention of precocious sister chromatid separation in meiosis I and for sister chromatid disjunction in meiosis II

Curr Genet. 2004 Aug;46(2):82-91. doi: 10.1007/s00294-004-0516-6. Epub 2004 Jul 6.

Authors

Santanu Kumar Ghosh¹, Soumitra Sau, Sudeshna Lahiri, Anuradha Lohia, Pratima Sinha

Affiliation

¹ Department of Biochemistry, Bose Institute, Kolkata, 700 054, India.

PMID: 15241623
DOI: 10.1007/s00294-004-0516-6

Abstract

The mitotic kinetochore of the budding yeast contains a number of proteins which are required for chromosome transmission but are non-essential for vegetative growth. We show that one such protein, Iml3, is essential for meiosis, in that the absence of this protein results in reduced spore viability, precocious sister chromatid segregation of artificial and natural chromosomes in meiosis I and chromosome non-disjunction in meiosis II.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Chromosome Segregation / physiology*
Crosses, Genetic
Cytoskeletal Proteins / genetics
Cytoskeletal Proteins / physiology*
Indoles
Meiosis / physiology*
Microscopy, Fluorescence
Plasmids / genetics
Saccharomyces cerevisiae Proteins / genetics
Saccharomyces cerevisiae Proteins / physiology*
Saccharomycetales / genetics
Saccharomycetales / physiology*
Sister Chromatid Exchange / physiology*
Spores, Fungal / physiology

Substances

Cytoskeletal Proteins
IML3 protein, S cerevisiae
Indoles
Saccharomyces cerevisiae Proteins
DAPI