Abstract
The yeast Hat1p/Hat2p type B histone acetyltransferase complex is localized to both the cytoplasm and nucleus. We isolate the nuclear form of the Hat1p/Hat2p complex and find that it copurifies with the product of the uncharacterized open reading frame YLL022C (named Hif1p). The functional significance of the association of Hif1p with the Hat1p/Hat2p complex is confirmed by the observation that hif1Delta and hat1Delta strains display similar defects in telomeric silencing and DNA double-strand break repair. Hif1p is a histone chaperone that selectively interacts with histones H3 and H4. Hif1p is also a chromatin assembly factor, promoting the deposition of histones in the presence of a yeast cytosolic extract. In vivo, the nuclear Hat1p/Hat2p/Hif1p complex is bound to acetylated histone H4, as well as histone H3. The association of Hif1p with acetylated H4 requires Hat1p and Hat2p providing a link between type B histone acetyltransferases and chromatin assembly.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Acetyltransferases / genetics
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Acetyltransferases / isolation & purification
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Acetyltransferases / metabolism*
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Alleles
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Blotting, Western
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Cell Extracts
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Cell Nucleus / chemistry
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Cell Nucleus / enzymology*
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Chromatin / metabolism*
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Cytoplasm / chemistry
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DNA Damage
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DNA Repair
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DNA-Binding Proteins / chemistry
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DNA-Binding Proteins / metabolism
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Electrophoresis, Polyacrylamide Gel
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Escherichia coli / metabolism
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Fluorescent Antibody Technique, Indirect
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Gene Deletion
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Gene Silencing
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Genes, Reporter
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Histone Acetyltransferases
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Hypoxia-Inducible Factor 1
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Models, Molecular
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Molecular Chaperones / metabolism
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Molecular Weight
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Nuclear Proteins / chemistry
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Nuclear Proteins / metabolism
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Open Reading Frames
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Saccharomyces cerevisiae / enzymology*
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae Proteins*
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Subcellular Fractions
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Substrate Specificity
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Telomere / metabolism
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Transcription Factors*
Substances
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Cell Extracts
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Chromatin
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DNA-Binding Proteins
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Hypoxia-Inducible Factor 1
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Molecular Chaperones
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Nuclear Proteins
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Saccharomyces cerevisiae Proteins
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Transcription Factors
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Acetyltransferases
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Histone Acetyltransferases