Abstract
Fission yeast ptr1-1 is one of the mRNA transport mutants that accumulate poly(A)+ RNA in the nuclei at the nonpermissive temperature. We found that the ptr1+ gene encodes a homolog of Saccharomyces cerevisiae Tom1p, a hect type ubiquitin ligase. In ptr1-1, a conserved amino acid in the hect domain of Ptr1p is mutated. The ptr1+ gene is essential for growth and its mutation did not affect nuclear protein export. A ptr1-1 rae1-167 double mutant showed a synthetic effect on a growth defect, indicating that Ptr1p functionally interacts with an essential mRNA export factor Rae1p. We also isolated a multi-copy suppressor for ptr1-1 and found that it is the mpd2+ gene isolated as a multi-copy suppressor of cdc7-PD1.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Active Transport, Cell Nucleus
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Amino Acid Sequence
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Cell Nucleus / metabolism*
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Cloning, Molecular
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Cosmids / genetics
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Diploidy
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Genes, Suppressor
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Homeodomain Proteins / genetics*
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Molecular Sequence Data
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Mutation
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Protein Structure, Tertiary
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RNA, Messenger / metabolism*
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Recombinant Proteins / genetics
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Recombinant Proteins / metabolism
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Saccharomyces cerevisiae / enzymology
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae Proteins / genetics*
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Schizosaccharomyces / enzymology
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Schizosaccharomyces / genetics*
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Schizosaccharomyces / growth & development
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Schizosaccharomyces / metabolism
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Schizosaccharomyces pombe Proteins / genetics
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Schizosaccharomyces pombe Proteins / metabolism
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Sequence Homology, Amino Acid
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Ubiquitin-Protein Ligases / genetics*
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Ubiquitin-Protein Ligases / metabolism*
Substances
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Homeodomain Proteins
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RNA, Messenger
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Recombinant Proteins
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Saccharomyces cerevisiae Proteins
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Schizosaccharomyces pombe Proteins
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TOM1 protein, S cerevisiae
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Ubiquitin-Protein Ligases