Rax1, a protein required for the establishment of the bipolar budding pattern in yeast

Atsushi Fujita; Matthew Lord; Takatoshi Hiroko; Fumika Hiroko; Tracy Chen; Chitoshi Oka; Yoshio Misumi; John Chant

doi:10.1016/j.gene.2003.11.021

Rax1, a protein required for the establishment of the bipolar budding pattern in yeast

Gene. 2004 Mar 3;327(2):161-9. doi: 10.1016/j.gene.2003.11.021.

Authors

Atsushi Fujita¹, Matthew Lord, Takatoshi Hiroko, Fumika Hiroko, Tracy Chen, Chitoshi Oka, Yoshio Misumi, John Chant

Affiliation

¹ National Institute of Advanced Industrial Science and Technology, Tsukuba Central 6-4, 1-1 Higashi, Tsukuba 305-8566, Japan. a-fujita@aist.go.jp

PMID: 14980713
DOI: 10.1016/j.gene.2003.11.021

Abstract

In Saccharomyces cerevisiae, cell type determines two distinct spatial budding patterns. Haploid cells exhibit an axial pattern, whereas diploid cells exhibit a bipolar pattern. Axl1, a member of the insulin-degrading enzyme (IDE) family, is the key morphological determinant for the haploid axial pattern. Here we identified a novel gene, RAX1, specifically required for the bipolar budding pattern. Loss of RAX1 alters the bipolar pattern of axl1 haploids resulting in reversion to the axial pattern, and also alters the bipolar patterns of bud3 and bud4 haploids. However, bud10 rax1 haploids exhibit a random budding pattern, suggesting Bud10 acts as the key proximal landmark in axial budding. Rax1 is required for the localization of Bud8, the distal bipolar budding landmark. Interestingly, Rax1 contains a C-terminal domain possessing some similarity to insulin-related peptides. Our results suggest that Rax1 is necessary for the establishment of the bipolar budding landmark.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Cell Division / genetics
Diploidy
Green Fluorescent Proteins
Luminescent Proteins / genetics
Luminescent Proteins / metabolism
Membrane Glycoproteins / genetics
Membrane Glycoproteins / metabolism
Membrane Proteins
Metalloendopeptidases
Microscopy, Fluorescence
Molecular Sequence Data
Mutation
Receptors, Cell Surface / genetics
Receptors, Cell Surface / metabolism
Recombinant Fusion Proteins / genetics
Recombinant Fusion Proteins / metabolism
Saccharomyces cerevisiae / genetics*
Saccharomyces cerevisiae / growth & development
Saccharomyces cerevisiae Proteins / genetics*
Saccharomyces cerevisiae Proteins / metabolism
Sequence Alignment
Sequence Homology, Amino Acid
Suppression, Genetic / genetics

Substances

BUD10 protein, S cerevisiae
BUD8 protein, S cerevisiae
BUD9 protein, S cerevisiae
Luminescent Proteins
Membrane Glycoproteins
Membrane Proteins
RAX1 protein, S cerevisiae
Receptors, Cell Surface
Recombinant Fusion Proteins
Saccharomyces cerevisiae Proteins
Green Fluorescent Proteins
AXL1 protein, S cerevisiae
Metalloendopeptidases