Abstract
Growth factors and morphogens need to be secreted to act on distant cells during development and in response to injury. Here, we report evidence that efficient export of a fibroblast growth factor (FGF), EGL-17, from the Caenorhabditis elegans developing vulva requires the lipoprotein receptor-related proteins Ce-LRP-1 and Ce-LRP-2 and a cytoplasmic adaptor protein, Ce-DAB-1 (Disabled). Lipoprotein receptors are transmembrane proteins best known for their roles in endocytosis. Ce-LRP-1 and Ce-LRP-2 possess a conserved intraluminal domain that can bind to EGL-17, as well as a cytosolic FXNPXY motif that can bind to Ce-DAB-1. Ce-DAB-1 contains signals that confer subcellular localization to Golgi-proximal vesicles. These results suggest a model in which Ce-DAB-1 coordinates selection of receptors and cargo, including EGL-17, for transport through the secretory pathway.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adaptor Proteins, Signal Transducing
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Adaptor Proteins, Vesicular Transport / metabolism
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Amino Acid Motifs
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Animals
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Apoptosis Regulatory Proteins
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Caenorhabditis elegans / cytology
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Caenorhabditis elegans / genetics*
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Caenorhabditis elegans / growth & development*
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Caenorhabditis elegans Proteins / genetics
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Caenorhabditis elegans Proteins / metabolism*
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Cell Movement
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Cytoplasm
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Female
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Fibroblast Growth Factors / metabolism*
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Fibroblast Growth Factors / pharmacology
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Fluorescent Antibody Technique
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Gene Silencing
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Golgi Apparatus
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Green Fluorescent Proteins
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Intercellular Signaling Peptides and Proteins / genetics
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Intercellular Signaling Peptides and Proteins / metabolism*
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Luminescent Proteins / metabolism
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Mutation
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Phosphoproteins / metabolism
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Precipitin Tests
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Receptors, Lipoprotein / metabolism*
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Saccharomyces cerevisiae
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Subcellular Fractions
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Two-Hybrid System Techniques
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Vulva / cytology
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Vulva / growth & development*
Substances
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Adaptor Proteins, Signal Transducing
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Adaptor Proteins, Vesicular Transport
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Apoptosis Regulatory Proteins
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Caenorhabditis elegans Proteins
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Dab2 protein, mouse
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Egl-17 protein, C elegans
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Intercellular Signaling Peptides and Proteins
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Luminescent Proteins
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Phosphoproteins
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Receptors, Lipoprotein
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Green Fluorescent Proteins
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Fibroblast Growth Factors