Abstract
The protein import machinery of the peroxisome consists of many proteins, collectively called the peroxins. By applying the split-ubiquitin technique we systematically tested the pair-wise interactions between the Nub- and Cub-labeled peroxins for the first time in the living cells of the yeast Saccharomyces cerevisiae. We found that Pex10p plays a central role in the protein interaction network by connecting the ubiquitin conjugation enzyme Pex4p to the other members of the protein import machinery. A yeast strain harboring a deletion of PEX3 enabled us to estimate the influence of the peroxisomal membrane on the formation of a subset of the investigated protein-protein interactions.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Biological Transport
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Cloning, Molecular
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Fungal Proteins / metabolism*
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Intracellular Membranes / metabolism
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Membrane Proteins / metabolism*
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Models, Molecular
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Peroxins
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Peroxisomal Biogenesis Factor 2
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Peroxisomes / metabolism*
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Protein Binding
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Protein Interaction Mapping
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Receptors, Cytoplasmic and Nuclear / metabolism*
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Saccharomyces cerevisiae / metabolism*
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Saccharomyces cerevisiae Proteins / metabolism*
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Ubiquitin-Protein Ligases
Substances
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Fungal Proteins
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Membrane Proteins
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PEX10 protein, S cerevisiae
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PEX12 protein, S cerevisiae
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PEX3 protein, S cerevisiae
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PEX4 protein, S cerevisiae
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Peroxins
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Receptors, Cytoplasmic and Nuclear
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Saccharomyces cerevisiae Proteins
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Peroxisomal Biogenesis Factor 2
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Ubiquitin-Protein Ligases