Characterization of vps33+, a gene required for vacuolar biogenesis and protein sorting in Schizosaccharomyces pombe

Yeast. 2003 Jul 30;20(10):845-55. doi: 10.1002/yea.1011.

Abstract

From the fission yeast Schizosaccharomyces pombe we have identified and deleted vps33, a gene encoding a homologue of VPS33, which is required for vacuolar biogenesis in S. cerevisiae cells. When the vps33(+) gene is disrupted, Sz. pombe strains are temperature-sensitive for growth and contain numerous small vesicular structures stained with FM4-64 in the cells. Deletion of the Sz. pombe vps33(+) gene results in pleiotropic phenotypes consistent with the absence of normal vacuoles, including missorting of vacuolar carboxypeptidase Y, various ion- and drug-sensitivities, and sporulation defects. These results are consistent with Vps33p being necessary for the morphogenesis of vacuoles and subsequent expression of vacuolar functions in Sz. pombe cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Blotting, Western
  • Carboxypeptidases / genetics
  • Carboxypeptidases / metabolism
  • Cathepsin A
  • Genes, Fungal / genetics
  • Genes, Fungal / physiology*
  • Genetic Complementation Test
  • Microscopy, Fluorescence
  • Molecular Sequence Data
  • Mutagenesis, Insertional
  • Protein Transport / genetics
  • Protein Transport / physiology
  • Pyridinium Compounds / metabolism
  • Quaternary Ammonium Compounds / metabolism
  • Schizosaccharomyces / genetics*
  • Schizosaccharomyces / metabolism
  • Schizosaccharomyces / ultrastructure
  • Schizosaccharomyces pombe Proteins / genetics
  • Schizosaccharomyces pombe Proteins / metabolism*
  • Sequence Homology, Amino Acid
  • Vacuoles / genetics*
  • Vacuoles / metabolism

Substances

  • FM 4-64
  • Pyridinium Compounds
  • Quaternary Ammonium Compounds
  • Schizosaccharomyces pombe Proteins
  • Carboxypeptidases
  • Cathepsin A