Bsp1p/Ypr171p is an adapter that directly links some synaptojanin family members to the cortical actin cytoskeleton in yeast

FEBS Lett. 2003 Feb 27;537(1-3):35-41. doi: 10.1016/s0014-5793(03)00067-x.

Abstract

In this study we identified a novel protein, Bsp1p, that interacts directly with two yeast synaptojanins, Sjl2p and Sjl3p, but not with Sjl1p. The interaction takes place via the Sac1/polyphosphoinositide phosphatase domain, whose conserved C-terminal region is important for binding. Subcellular localization and genetic interactions revealed a function of Bsp1p in the cortical actin cytoskeleton. A fraction of Bsp1p was found to be membrane-associated. Studies with mutants of phosphatidylinositol 4-kinase, PIK1, suggested that the interaction with membranes is facilitated by phosphoinositides. We propose that Bsp1p is an adapter that links Sjl2p and Sjl3p to the cortical actin cytoskeleton.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actins / metabolism*
  • Adaptor Proteins, Vesicular Transport / genetics*
  • Adaptor Proteins, Vesicular Transport / metabolism
  • Base Sequence
  • Cytoskeleton / metabolism*
  • DNA Primers
  • Genotype
  • Kinetics
  • Molecular Sequence Data
  • Open Reading Frames
  • Phosphatidylinositols / metabolism
  • Phosphoric Monoester Hydrolases / genetics*
  • Plasmids
  • Saccharomyces cerevisiae / enzymology
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae Proteins / genetics*
  • Saccharomyces cerevisiae Proteins / metabolism

Substances

  • Actins
  • Adaptor Proteins, Vesicular Transport
  • Bsp1 protein, S cerevisiae
  • DNA Primers
  • Phosphatidylinositols
  • Saccharomyces cerevisiae Proteins
  • INP51 protein, S cerevisiae
  • INP52 protein, S cerevisiae
  • Phosphoric Monoester Hydrolases