Abstract
Sgt1p is a highly conserved eucaryotic protein that is required for both SCF (Skp1p/Cdc53p-Cullin-F-box)-mediated ubiquitination and kinetochore function in yeast. We show here that Sgtlp is also involved in the cyclic AMP (cAMP) pathway in Saccharomyces cerevisiae. SGT1 is an allele-specific suppressor of cdc35-1, a thermosensitive mutation in the leucine-rich repeat domain of the adenylyl cyclase Cyrlp/Cdc35p. We demonstrate that Sgt1p and Cyrlp/Cdc35p physically interact and that the activity of the cAMP pathway is affected in an sgt1 conditional mutant. Sequence analysis suggests that Sgtlp has features of a cochaperone. Thus, Sgt1p is a novel activator of adenylyl cyclase in S. cerevisiae and may function in the assembly or the conformational activation of specific multiprotein complexes.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adaptor Proteins, Signal Transducing
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Adenylyl Cyclases / chemistry
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Adenylyl Cyclases / genetics
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Adenylyl Cyclases / metabolism*
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Alleles
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Amino Acid Sequence
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Base Sequence
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Cyclic AMP / metabolism*
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DNA, Fungal / genetics
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Enzyme Activation
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Genes, Fungal
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Models, Molecular
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Molecular Chaperones / chemistry
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Molecular Chaperones / genetics
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Molecular Chaperones / metabolism
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Molecular Sequence Data
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Mutation
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Protein Structure, Tertiary
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Repressor Proteins / chemistry
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Repressor Proteins / genetics
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Repressor Proteins / metabolism*
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae / metabolism*
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Saccharomyces cerevisiae Proteins / chemistry
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Saccharomyces cerevisiae Proteins / genetics
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Saccharomyces cerevisiae Proteins / metabolism*
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Sequence Homology, Amino Acid
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Temperature
Substances
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Adaptor Proteins, Signal Transducing
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DNA, Fungal
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Molecular Chaperones
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Repressor Proteins
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SGT1 protein, S cerevisiae
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Saccharomyces cerevisiae Proteins
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Cyclic AMP
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Adenylyl Cyclases