Multiple associated proteins regulate proteasome structure and function

Mol Cell. 2002 Sep;10(3):495-507. doi: 10.1016/s1097-2765(02)00638-x.

Abstract

We have identified proteins that are abundant in affinity-purified proteasomes, but absent from proteasomes as previously defined because elevated salt concentrations dissociate them during purification. The major components are a deubiquitinating enzyme (Ubp6), a ubiquitin-ligase (Hul5), and an uncharacterized protein (Ecm29). Ecm29 tethers the proteasome core particle to the regulatory particle. Proteasome binding activates Ubp6 300-fold and is mediated by the ubiquitin-like domain of Ubp6, which is required for function in vivo. Ubp6 recognizes the proteasome base and its subunit Rpn1, suggesting that proteasome binding positions Ubp6 proximally to the substrate translocation channel. ubp6Delta mutants exhibit accelerated turnover of ubiquitin, indicating that deubiquitination events catalyzed by Ubp6 prevent translocation of ubiquitin into the proteolytic core particle.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenosine Triphosphate / metabolism
  • Binding Sites
  • Canavanine / metabolism
  • Cysteine Endopeptidases / chemistry*
  • Cysteine Endopeptidases / isolation & purification
  • Cysteine Endopeptidases / metabolism*
  • Cysteine Endopeptidases / ultrastructure
  • Endopeptidases / genetics
  • Endopeptidases / isolation & purification
  • Endopeptidases / metabolism*
  • Ligases / genetics
  • Ligases / isolation & purification
  • Ligases / metabolism
  • Multienzyme Complexes / chemistry*
  • Multienzyme Complexes / isolation & purification
  • Multienzyme Complexes / metabolism*
  • Multienzyme Complexes / ultrastructure
  • Proteasome Endopeptidase Complex
  • Protein Binding
  • Protein Subunits / genetics
  • Protein Subunits / isolation & purification
  • Protein Subunits / metabolism
  • Proteins / genetics
  • Proteins / metabolism
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Saccharomyces cerevisiae / metabolism
  • Saccharomyces cerevisiae Proteins / genetics
  • Saccharomyces cerevisiae Proteins / isolation & purification
  • Saccharomyces cerevisiae Proteins / metabolism*
  • Salts / chemistry
  • Ubiquitin-Protein Ligases*

Substances

  • Multienzyme Complexes
  • PA700 proteasome activator
  • Protein Subunits
  • Proteins
  • Recombinant Fusion Proteins
  • Saccharomyces cerevisiae Proteins
  • Salts
  • Canavanine
  • Adenosine Triphosphate
  • HUL5 protein, S cerevisiae
  • Ubiquitin-Protein Ligases
  • Endopeptidases
  • Cysteine Endopeptidases
  • Proteasome Endopeptidase Complex
  • UBP6 protein, S cerevisiae
  • Ligases