Abstract
Proteins involved in chromosome segregation during mitosis are likely to participate in other cell cycle-coordinated processes. Using a two-hybrid screen we identified a novel nuclear protein, Lin1, interacting with Irr1p/Scc3p, a component of the cohesin complex. The second round of two-hybrid assay with Lin1p as the bait resulted in the identification of six proteins: Prp8, Slx5, Siz2, Wss1, Rfc1 and YIL149w. These proteins have previously been shown to participate in mRNA splicing, DNA replication, chromosome condensation, chromatid separation and alternative cohesion. We propose that Lin1p may constitute a link among these processes.
Copyright 2002 John Wiley & Sons, Ltd.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Cell Cycle Proteins
-
Chromatids
-
Chromosomal Proteins, Non-Histone
-
Chromosome Segregation*
-
Cohesins
-
DNA Replication*
-
Fungal Proteins / metabolism
-
Intracellular Signaling Peptides and Proteins
-
Nuclear Proteins / genetics
-
Nuclear Proteins / metabolism*
-
RNA Splicing*
-
Saccharomyces cerevisiae / genetics*
-
Saccharomyces cerevisiae / metabolism
-
Saccharomyces cerevisiae Proteins / genetics
-
Saccharomyces cerevisiae Proteins / metabolism*
-
Sister Chromatid Exchange
-
Two-Hybrid System Techniques
Substances
-
Cell Cycle Proteins
-
Chromosomal Proteins, Non-Histone
-
Fungal Proteins
-
IRR1 protein, S cerevisiae
-
Intracellular Signaling Peptides and Proteins
-
Lin1 protein, S cerevisiae
-
Nuclear Proteins
-
Saccharomyces cerevisiae Proteins