Proteins interacting with Lin 1p, a putative link between chromosome segregation, mRNA splicing and DNA replication in Saccharomyces cerevisiae

Yeast. 2002 Nov;19(15):1323-33. doi: 10.1002/yea.919.

Abstract

Proteins involved in chromosome segregation during mitosis are likely to participate in other cell cycle-coordinated processes. Using a two-hybrid screen we identified a novel nuclear protein, Lin1, interacting with Irr1p/Scc3p, a component of the cohesin complex. The second round of two-hybrid assay with Lin1p as the bait resulted in the identification of six proteins: Prp8, Slx5, Siz2, Wss1, Rfc1 and YIL149w. These proteins have previously been shown to participate in mRNA splicing, DNA replication, chromosome condensation, chromatid separation and alternative cohesion. We propose that Lin1p may constitute a link among these processes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Cycle Proteins
  • Chromatids
  • Chromosomal Proteins, Non-Histone
  • Chromosome Segregation*
  • Cohesins
  • DNA Replication*
  • Fungal Proteins / metabolism
  • Intracellular Signaling Peptides and Proteins
  • Nuclear Proteins / genetics
  • Nuclear Proteins / metabolism*
  • RNA Splicing*
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / metabolism
  • Saccharomyces cerevisiae Proteins / genetics
  • Saccharomyces cerevisiae Proteins / metabolism*
  • Sister Chromatid Exchange
  • Two-Hybrid System Techniques

Substances

  • Cell Cycle Proteins
  • Chromosomal Proteins, Non-Histone
  • Fungal Proteins
  • IRR1 protein, S cerevisiae
  • Intracellular Signaling Peptides and Proteins
  • Lin1 protein, S cerevisiae
  • Nuclear Proteins
  • Saccharomyces cerevisiae Proteins