Abstract
Ypt1p regulates vesicle tethering and fusion events from the ER to the Golgi and through the early Golgi. Genetic studies have suggested a functional relationship between Ypt1p and Ypt31p/Ypt32p. Ypt31p and Ypt32p are a pair of functionally redundant GTPases that act after Ypt1p to mediate intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi. Here we report that a novel Ypt32p exchange factor is a putative effector of Ypt1p. These findings implicate small GTP-binding proteins of the Ypt/Rab family in a signal cascade that directs membrane traffic through the secretory pathway.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Carrier Proteins / metabolism
-
Cell Membrane / metabolism
-
Cytosol / metabolism
-
Golgi Apparatus / metabolism
-
Guanosine Triphosphate / metabolism
-
Protein Binding
-
Recombinant Fusion Proteins / metabolism
-
Saccharomyces cerevisiae / metabolism*
-
Saccharomyces cerevisiae Proteins / metabolism
-
Signal Transduction
-
Subcellular Fractions / metabolism
-
Time Factors
-
Vesicular Transport Proteins*
-
rab GTP-Binding Proteins / metabolism*
Substances
-
Carrier Proteins
-
Recombinant Fusion Proteins
-
Saccharomyces cerevisiae Proteins
-
USO1 protein, S cerevisiae
-
Vesicular Transport Proteins
-
Guanosine Triphosphate
-
YPT1 protein, S cerevisiae
-
YPT32 protein, S cerevisiae
-
rab GTP-Binding Proteins