A Ypt32p exchange factor is a putative effector of Ypt1p

Mol Biol Cell. 2002 Sep;13(9):3336-43. doi: 10.1091/mbc.01-12-0577.

Abstract

Ypt1p regulates vesicle tethering and fusion events from the ER to the Golgi and through the early Golgi. Genetic studies have suggested a functional relationship between Ypt1p and Ypt31p/Ypt32p. Ypt31p and Ypt32p are a pair of functionally redundant GTPases that act after Ypt1p to mediate intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi. Here we report that a novel Ypt32p exchange factor is a putative effector of Ypt1p. These findings implicate small GTP-binding proteins of the Ypt/Rab family in a signal cascade that directs membrane traffic through the secretory pathway.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carrier Proteins / metabolism
  • Cell Membrane / metabolism
  • Cytosol / metabolism
  • Golgi Apparatus / metabolism
  • Guanosine Triphosphate / metabolism
  • Protein Binding
  • Recombinant Fusion Proteins / metabolism
  • Saccharomyces cerevisiae / metabolism*
  • Saccharomyces cerevisiae Proteins / metabolism
  • Signal Transduction
  • Subcellular Fractions / metabolism
  • Time Factors
  • Vesicular Transport Proteins*
  • rab GTP-Binding Proteins / metabolism*

Substances

  • Carrier Proteins
  • Recombinant Fusion Proteins
  • Saccharomyces cerevisiae Proteins
  • USO1 protein, S cerevisiae
  • Vesicular Transport Proteins
  • Guanosine Triphosphate
  • YPT1 protein, S cerevisiae
  • YPT32 protein, S cerevisiae
  • rab GTP-Binding Proteins