Abstract
The genome of Saccharomyces cerevisia encodes four mismatch repair MutL proteins and these proteins form three heterocomplexes: Mlh1-Mlh2, Mlh1-Mlh3, and Mlh1-Pms1. Only, the Mlh1-Mlh3 heterocomplex has been implicated specifically in promotion of meiotic crossing-over. In this report, we show that yeast Mlh3 co-immunoprecipitates with Sgs1 helicase in sporulating cells at late stage of meiotic prophase I. Sgs1, a member of the RecQ DNA helicase family, appears to form a stable complex with topoisomerase III (Top3) during meiosis. We suggest that Mlh1-Mlh3 heterocomplex may act as a molecular matchmaker to coordinate Sgs1-Top3 complex in the resolution of meiotic recombination intermediates.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adaptor Proteins, Signal Transducing
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Blotting, Western
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Carrier Proteins / metabolism*
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DNA Helicases / metabolism*
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DNA Topoisomerases, Type I / metabolism*
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Fungal Proteins / metabolism*
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Meiosis*
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MutL Protein Homolog 1
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MutL Proteins
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Polymerase Chain Reaction
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Precipitin Tests
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Prophase
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Protein Binding
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RecQ Helicases
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Recombination, Genetic
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Saccharomyces cerevisiae / enzymology
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Saccharomyces cerevisiae / metabolism*
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Saccharomyces cerevisiae Proteins*
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Time Factors
Substances
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Adaptor Proteins, Signal Transducing
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Carrier Proteins
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Fungal Proteins
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MLH1 protein, S cerevisiae
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MLH3 protein, S cerevisiae
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Saccharomyces cerevisiae Proteins
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SGS1 protein, S cerevisiae
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MutL Protein Homolog 1
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MutL Proteins
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DNA Helicases
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RecQ Helicases
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DNA Topoisomerases, Type I