Carnitine uptake by AGP2 in yeast Saccharomyces cerevisiae is dependent on Hog1 MAP kinase pathway

Jiyoung Lee; Boyoung Lee; Dongjin Shin; Sang-Soo Kwak; Jeong Dong Bahk; Chae Oh Lim; Dae-Jin Yun

Carnitine uptake by AGP2 in yeast Saccharomyces cerevisiae is dependent on Hog1 MAP kinase pathway

Mol Cells. 2002 Jun 30;13(3):407-12.

Authors

Jiyoung Lee¹, Boyoung Lee, Dongjin Shin, Sang-Soo Kwak, Jeong Dong Bahk, Chae Oh Lim, Dae-Jin Yun

Affiliation

¹ Division of Applied Life Science, Plant Molecular Biology and Biotechnology Research Center, Graduate School of Gyeongsang National University, Jinju, Korea.

PMID: 12132580

Abstract

The AGP2 gene encodes a plasma membrane carnitine transporter in S. cerevisiae. Here, we report the identification of AGP2 as an osmotic stress response gene. AGP2 was isolated from mTn3 tagged mutants that contained in-frame fusions with lacZ. The expression of AGP2 was down-regulated by osmotic stresses, including NaCl, sorbitol, and KCI. We also found that carnitine uptake was inhibited by NaCl. In the ssk1delta stelldelta double-mutant strain, the expression of AGP2 and the uptake of carnitine were greatly reduced compared to the wild-type strain. Furthermore, carnitine uptake was inhibited by the constitutive expression of PBS2, which encodes a MAPKK that activates Hog1. We concluded, therefore, that the HOG pathway plays an important role in the regulation of carnitine uptake in S. cerevisiae.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Transport Systems / antagonists & inhibitors
Amino Acid Transport Systems / genetics
Amino Acid Transport Systems / isolation & purification
Amino Acid Transport Systems / metabolism*
Carnitine / antagonists & inhibitors*
Carnitine / pharmacokinetics*
Down-Regulation / drug effects
Mitogen-Activated Protein Kinase Kinases / pharmacology
Mitogen-Activated Protein Kinases / metabolism
Mitogen-Activated Protein Kinases / physiology*
Mutation
Saccharomyces cerevisiae / metabolism*
Saccharomyces cerevisiae Proteins / antagonists & inhibitors
Saccharomyces cerevisiae Proteins / genetics
Saccharomyces cerevisiae Proteins / isolation & purification
Saccharomyces cerevisiae Proteins / metabolism*
Saccharomyces cerevisiae Proteins / physiology*
Sodium Chloride / pharmacology
Symporters / antagonists & inhibitors
Symporters / genetics
Symporters / isolation & purification
Symporters / metabolism*

Substances

Agp2 protein, S cerevisiae
Amino Acid Transport Systems
Saccharomyces cerevisiae Proteins
Symporters
Sodium Chloride
HOG1 protein, S cerevisiae
Mitogen-Activated Protein Kinases
Mitogen-Activated Protein Kinase Kinases
PBS2 protein, S cerevisiae
Carnitine