Abstract
In Caenorhabditis elegans, Ras/ERK and Wnt/beta-catenin signaling pathways cooperate to induce P12 and vulval cell fates in a Hox-dependent manner. Here we describe eor-1 and eor-2, two new positively acting nuclear components of the Ras and Wnt pathways. eor-1 and eor-2 act downstream or in parallel to ERK and function redundantly with the Mediator complex gene sur-2 and the functionally related gene lin-25, such that removal of both eor-1/eor-2 and sur-2/lin-25 mimics the removal of a main Ras pathway component. Furthermore, the eor-1 and eor-2 mutant backgrounds reveal an essential role for the Elk1-related gene lin-1. eor-1 and eor-2 also act downstream or in parallel to pry-1 Axin and therefore act at the convergence of the Ras and Wnt pathways. eor-1 encodes the ortholog of human PLZF, a BTB/zinc-finger transcription factor that is fused to RARalpha in acute promyelocytic leukemia. eor-2 encodes a novel protein. EOR-1/PLZF and EOR-2 appear to function closely together and cooperate with Hox genes to promote the expression of Ras- and Wnt-responsive genes. Further studies of eor-1 and eor-2 may provide insight into the roles of PLZF in normal development and leukemogenesis.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Caenorhabditis elegans / genetics
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Caenorhabditis elegans / metabolism
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Caenorhabditis elegans Proteins / genetics
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Caenorhabditis elegans Proteins / metabolism*
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Cell Nucleus / metabolism
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Cytoskeletal Proteins / genetics
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Cytoskeletal Proteins / metabolism
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DNA, Helminth
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism*
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Green Fluorescent Proteins
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Helminth Proteins / genetics
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Helminth Proteins / metabolism*
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Homeodomain Proteins / genetics
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Homeodomain Proteins / metabolism
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Luminescent Proteins / genetics
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Luminescent Proteins / metabolism
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Mitogen-Activated Protein Kinase 1
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Molecular Sequence Data
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Nuclear Proteins / genetics
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Nuclear Proteins / metabolism*
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Protein Kinases / genetics
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Protein Kinases / metabolism
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Protein Serine-Threonine Kinases / genetics
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Protein Serine-Threonine Kinases / metabolism*
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Proto-Oncogene Proteins / genetics
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Proto-Oncogene Proteins / metabolism
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
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Signal Transduction*
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Transcription Factors / genetics
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Transcription Factors / metabolism*
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Up-Regulation
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Wnt Proteins
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Zebrafish Proteins*
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Zinc Fingers*
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ras Proteins / genetics
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ras Proteins / metabolism*
Substances
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Caenorhabditis elegans Proteins
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Cytoskeletal Proteins
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DNA, Helminth
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DNA-Binding Proteins
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EOR-1 protein, C elegans
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EOR-2 protein, C elegans
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Helminth Proteins
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Homeodomain Proteins
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LIN-25 protein, C elegans
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Lin-1 protein, C elegans
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Luminescent Proteins
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Nuclear Proteins
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Proto-Oncogene Proteins
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Pry-1 protein, C elegans
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Recombinant Fusion Proteins
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Sur-2 protein, C elegans
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Transcription Factors
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Wnt Proteins
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Zebrafish Proteins
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bar-1 protein, C elegans
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lin-39 protein, C elegans
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let-60 protein, C elegans
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Green Fluorescent Proteins
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Protein Kinases
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Protein Serine-Threonine Kinases
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Mitogen-Activated Protein Kinase 1
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mpk-1 protein, C elegans
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ras Proteins
Associated data
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GENBANK/AF519108
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GENBANK/AF519109
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GENBANK/AF519110