Abstract
Size homeostasis in budding yeast requires that cells grow to a critical size before commitment to division in the late prereplicative growth phase of the cell cycle, an event termed Start. We determined cell size distributions for the complete set of approximately 6000 Saccharomyces cerevisiae gene deletion strains and identified approximately 500 abnormally small (whi) or large (lge) mutants. Genetic analysis revealed a complex network of newly found factors that govern critical cell size at Start, the most potent of which were Sfp1, Sch9, Cdh1, Prs3, and Whi5. Ribosome biogenesis is intimately linked to cell size through Sfp1, a transcription factor that controls the expression of at least 60 genes implicated in ribosome assembly. Cell growth and division appear to be coupled by multiple conserved mechanisms.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Cdh1 Proteins
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Cell Cycle
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Cell Division*
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Cell Nucleolus / metabolism
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Crosses, Genetic
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / physiology*
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Epistasis, Genetic
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Gene Deletion
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Gene Expression Regulation, Fungal
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Genes, Essential
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Genes, Fungal*
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Mutation
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Oligonucleotide Array Sequence Analysis
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Oxygen Consumption
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Phenotype
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Protein Kinases / genetics
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Protein Kinases / physiology
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Ribosomes / metabolism*
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Saccharomyces cerevisiae / cytology*
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae / growth & development
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Saccharomyces cerevisiae / physiology*
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Saccharomyces cerevisiae / ultrastructure
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Saccharomyces cerevisiae Proteins / biosynthesis
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Saccharomyces cerevisiae Proteins / genetics
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Saccharomyces cerevisiae Proteins / physiology*
Substances
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CDH1 protein, S cerevisiae
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Cdh1 Proteins
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DNA-Binding Proteins
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SFP1 protein, S cerevisiae
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Saccharomyces cerevisiae Proteins
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Protein Kinases
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SCH9 protein kinase