Abstract
Fission yeast Cid13 and budding yeast Trf4/5 are members of a newly identified nucleotidyltransferase family conserved from yeast to man. Trf4/5 are thought to be essential DNA polymerases. We report that Cid13 is a poly(A) polymerase. Unlike conventional poly(A) polymerases, which act in the nucleus and indiscriminately polyadenylate all mRNA, Cid13 is a cytoplasmic enzyme that specifically targets suc22 mRNA that encodes a subunit of ribonucleotide reductase (RNR). cid13 mutants have reduced dNTP pools and are sensitive to hydroxyurea, an RNR inhibitor. We propose that Cid13 defines a cytoplasmic form of poly(A) polymerase important for DNA replication and genome maintenance.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Cell Nucleus / enzymology*
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Cell Nucleus / genetics
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Chromosomal Proteins, Non-Histone / genetics
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Chromosomal Proteins, Non-Histone / metabolism
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Cytoplasm / enzymology*
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Cytoplasm / genetics
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DNA / biosynthesis
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DNA / genetics
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DNA Replication / genetics*
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DNA-Directed DNA Polymerase / genetics
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DNA-Directed DNA Polymerase / metabolism
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DNA-Directed RNA Polymerases / genetics
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DNA-Directed RNA Polymerases / isolation & purification*
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DNA-Directed RNA Polymerases / metabolism
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Eukaryotic Cells / cytology
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Eukaryotic Cells / enzymology*
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Fungal Proteins / genetics
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Fungal Proteins / metabolism
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Gene Expression Regulation, Enzymologic / genetics
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Genetic Testing
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Genomic Library
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Hydroxyurea / pharmacology
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Molecular Sequence Data
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Mutation / drug effects
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Mutation / genetics
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Nuclear Proteins*
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Polynucleotide Adenylyltransferase / genetics
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Polynucleotide Adenylyltransferase / isolation & purification*
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Polynucleotide Adenylyltransferase / metabolism
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RNA, Messenger / genetics
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RNA, Messenger / metabolism*
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RNA-Binding Proteins / genetics
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RNA-Binding Proteins / metabolism
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Ribonucleotide Reductases / genetics
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Ribonucleotide Reductases / metabolism*
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Schizosaccharomyces / enzymology*
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Schizosaccharomyces / genetics
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Schizosaccharomyces pombe Proteins*
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Sequence Homology, Amino Acid
Substances
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Chromosomal Proteins, Non-Histone
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Fungal Proteins
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Nuclear Proteins
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RNA, Messenger
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RNA-Binding Proteins
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Schizosaccharomyces pombe Proteins
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DNA
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Ribonucleotide Reductases
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SUC22 protein, S pombe
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Cid13 protein, S pombe
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Polynucleotide Adenylyltransferase
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DNA-Directed RNA Polymerases
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DNA-Directed DNA Polymerase
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Hydroxyurea