Cid13 is a cytoplasmic poly(A) polymerase that regulates ribonucleotide reductase mRNA

Cell. 2002 May 31;109(5):563-73. doi: 10.1016/s0092-8674(02)00753-5.

Abstract

Fission yeast Cid13 and budding yeast Trf4/5 are members of a newly identified nucleotidyltransferase family conserved from yeast to man. Trf4/5 are thought to be essential DNA polymerases. We report that Cid13 is a poly(A) polymerase. Unlike conventional poly(A) polymerases, which act in the nucleus and indiscriminately polyadenylate all mRNA, Cid13 is a cytoplasmic enzyme that specifically targets suc22 mRNA that encodes a subunit of ribonucleotide reductase (RNR). cid13 mutants have reduced dNTP pools and are sensitive to hydroxyurea, an RNR inhibitor. We propose that Cid13 defines a cytoplasmic form of poly(A) polymerase important for DNA replication and genome maintenance.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Cell Nucleus / enzymology*
  • Cell Nucleus / genetics
  • Chromosomal Proteins, Non-Histone / genetics
  • Chromosomal Proteins, Non-Histone / metabolism
  • Cytoplasm / enzymology*
  • Cytoplasm / genetics
  • DNA / biosynthesis
  • DNA / genetics
  • DNA Replication / genetics*
  • DNA-Directed DNA Polymerase / genetics
  • DNA-Directed DNA Polymerase / metabolism
  • DNA-Directed RNA Polymerases / genetics
  • DNA-Directed RNA Polymerases / isolation & purification*
  • DNA-Directed RNA Polymerases / metabolism
  • Eukaryotic Cells / cytology
  • Eukaryotic Cells / enzymology*
  • Fungal Proteins / genetics
  • Fungal Proteins / metabolism
  • Gene Expression Regulation, Enzymologic / genetics
  • Genetic Testing
  • Genomic Library
  • Hydroxyurea / pharmacology
  • Molecular Sequence Data
  • Mutation / drug effects
  • Mutation / genetics
  • Nuclear Proteins*
  • Polynucleotide Adenylyltransferase / genetics
  • Polynucleotide Adenylyltransferase / isolation & purification*
  • Polynucleotide Adenylyltransferase / metabolism
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism*
  • RNA-Binding Proteins / genetics
  • RNA-Binding Proteins / metabolism
  • Ribonucleotide Reductases / genetics
  • Ribonucleotide Reductases / metabolism*
  • Schizosaccharomyces / enzymology*
  • Schizosaccharomyces / genetics
  • Schizosaccharomyces pombe Proteins*
  • Sequence Homology, Amino Acid

Substances

  • Chromosomal Proteins, Non-Histone
  • Fungal Proteins
  • Nuclear Proteins
  • RNA, Messenger
  • RNA-Binding Proteins
  • Schizosaccharomyces pombe Proteins
  • DNA
  • Ribonucleotide Reductases
  • SUC22 protein, S pombe
  • Cid13 protein, S pombe
  • Polynucleotide Adenylyltransferase
  • DNA-Directed RNA Polymerases
  • DNA-Directed DNA Polymerase
  • Hydroxyurea