The Sec34/Sec35p complex, a Ypt1p effector required for retrograde intra-Golgi trafficking, interacts with Golgi SNAREs and COPI vesicle coat proteins

J Cell Biol. 2002 May 13;157(4):631-43. doi: 10.1083/jcb.200111081. Epub 2002 May 13.

Abstract

The Sec34/35 complex was identified as one of the evolutionarily conserved protein complexes that regulates a cis-Golgi step in intracellular vesicular transport. We have identified three new proteins that associate with Sec35p and Sec34p in yeast cytosol. Mutations in these Sec34/35 complex subunits result in defects in basic Golgi functions, including glycosylation of secretory proteins, protein sorting, and retention of Golgi resident proteins. Furthermore, the Sec34/35 complex interacts genetically and physically with the Rab protein Ypt1p, intra-Golgi SNARE molecules, as well as with Golgi vesicle coat complex COPI. We propose that the Sec34/35 protein complex acts as a tether that connects cis-Golgi membranes and COPI-coated, retrogradely targeted intra-Golgi vesicles.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptor Proteins, Vesicular Transport*
  • COP-Coated Vesicles / metabolism*
  • COP-Coated Vesicles / ultrastructure
  • Carrier Proteins / genetics
  • Carrier Proteins / isolation & purification
  • Carrier Proteins / metabolism*
  • Coat Protein Complex I / genetics
  • Coat Protein Complex I / metabolism
  • Golgi Apparatus / metabolism*
  • Golgi Apparatus / ultrastructure
  • Intracellular Signaling Peptides and Proteins
  • Macromolecular Substances
  • Membrane Proteins / genetics
  • Membrane Proteins / metabolism*
  • Mutation / genetics
  • Peptides / genetics
  • Peptides / isolation & purification
  • Protein Binding / genetics
  • Protein Transport / genetics*
  • SNARE Proteins
  • Saccharomyces cerevisiae / cytology
  • Saccharomyces cerevisiae / metabolism
  • Saccharomyces cerevisiae Proteins / genetics
  • Saccharomyces cerevisiae Proteins / isolation & purification
  • Vesicular Transport Proteins*
  • rab GTP-Binding Proteins / genetics
  • rab GTP-Binding Proteins / metabolism*

Substances

  • Adaptor Proteins, Vesicular Transport
  • COG2 protein, S cerevisiae
  • COG3 protein, S cerevisiae
  • Carrier Proteins
  • Coat Protein Complex I
  • Intracellular Signaling Peptides and Proteins
  • Macromolecular Substances
  • Membrane Proteins
  • Peptides
  • SNARE Proteins
  • Saccharomyces cerevisiae Proteins
  • TFI3 protein, S cerevisiae
  • Vesicular Transport Proteins
  • YPT1 protein, S cerevisiae
  • rab GTP-Binding Proteins