Abstract
The Sec34/35 complex was identified as one of the evolutionarily conserved protein complexes that regulates a cis-Golgi step in intracellular vesicular transport. We have identified three new proteins that associate with Sec35p and Sec34p in yeast cytosol. Mutations in these Sec34/35 complex subunits result in defects in basic Golgi functions, including glycosylation of secretory proteins, protein sorting, and retention of Golgi resident proteins. Furthermore, the Sec34/35 complex interacts genetically and physically with the Rab protein Ypt1p, intra-Golgi SNARE molecules, as well as with Golgi vesicle coat complex COPI. We propose that the Sec34/35 protein complex acts as a tether that connects cis-Golgi membranes and COPI-coated, retrogradely targeted intra-Golgi vesicles.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adaptor Proteins, Vesicular Transport*
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COP-Coated Vesicles / metabolism*
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COP-Coated Vesicles / ultrastructure
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Carrier Proteins / genetics
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Carrier Proteins / isolation & purification
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Carrier Proteins / metabolism*
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Coat Protein Complex I / genetics
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Coat Protein Complex I / metabolism
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Golgi Apparatus / metabolism*
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Golgi Apparatus / ultrastructure
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Intracellular Signaling Peptides and Proteins
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Macromolecular Substances
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Membrane Proteins / genetics
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Membrane Proteins / metabolism*
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Mutation / genetics
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Peptides / genetics
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Peptides / isolation & purification
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Protein Binding / genetics
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Protein Transport / genetics*
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SNARE Proteins
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Saccharomyces cerevisiae / cytology
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Saccharomyces cerevisiae / metabolism
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Saccharomyces cerevisiae Proteins / genetics
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Saccharomyces cerevisiae Proteins / isolation & purification
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Vesicular Transport Proteins*
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rab GTP-Binding Proteins / genetics
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rab GTP-Binding Proteins / metabolism*
Substances
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Adaptor Proteins, Vesicular Transport
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COG2 protein, S cerevisiae
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COG3 protein, S cerevisiae
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Carrier Proteins
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Coat Protein Complex I
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Intracellular Signaling Peptides and Proteins
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Macromolecular Substances
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Membrane Proteins
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Peptides
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SNARE Proteins
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Saccharomyces cerevisiae Proteins
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TFI3 protein, S cerevisiae
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Vesicular Transport Proteins
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YPT1 protein, S cerevisiae
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rab GTP-Binding Proteins