Abstract
The newly discovered Saccharomyces cerevisiae gene KRR1 (YCL059c) encodes a protein essential for cell viability. Krr1p contains a motif of clustered basic amino acids highly conserved in the evolutionarly distant species from yeast to human. We demonstrate that Krr1p is localized in the nucleolus. The KRR1 gene is highly expressed in dividing cells and its expression ceases almost completely when cells enter the stationary phase. In vivo depletion of Krr1p leads to drastic reduction of 40S ribosomal subunits due to defective 18S rRNA synthesis. We propose that Krr1p is required for proper processing of pre-rRNA and the assembly of preribosomal 40S subunits.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Binding Sites
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Blotting, Northern
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Blotting, Western
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Cell Division
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Epitopes
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Fungal Proteins / genetics
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Fungal Proteins / physiology*
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Models, Genetic
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Plasmids / metabolism
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Polymerase Chain Reaction
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Polyribosomes / metabolism
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RNA / metabolism
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RNA, Ribosomal / metabolism*
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RNA, Ribosomal / physiology*
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RNA, Ribosomal, 18S / metabolism*
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RNA-Binding Proteins*
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Saccharomyces cerevisiae / metabolism*
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Saccharomyces cerevisiae Proteins*
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Time Factors
Substances
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Epitopes
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Fungal Proteins
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KRR1 protein, S cerevisiae
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RNA, Ribosomal
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RNA, Ribosomal, 18S
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RNA-Binding Proteins
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Saccharomyces cerevisiae Proteins
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RNA