The Saccharomyces cerevisiae Fin1 protein forms cell cycle-specific filaments between spindle pole bodies

Proc Natl Acad Sci U S A. 2002 Apr 16;99(8):5390-3. doi: 10.1073/pnas.072556099. Epub 2002 Apr 2.

Abstract

The FIN1 gene from the yeast Saccharomyces cerevisiae encodes a basic protein with putative coiled-coil regions. Here we show that in large-budded cells a green fluorescent protein-Fin1 fusion protein is visible as a filament between the two spindle pole bodies. In resting cells the protein is undetectable, and in small-budded cells it is localized in the nucleus. During late mitosis it localizes on the spindle pole bodies. Filaments of cyano fluorescent protein-tagged Fin1 colocalize with filaments of green fluorescent protein-tagged Tub1 only in large-budded cells. By electron and atomic force microscopy we showed that purified recombinant Fin1p self-assembles into filaments with a diameter of approximately 10 nm. Our results indicate that the Fin1 protein forms a cell cycle-specific filament, additional to the microtubules, between the spindle pole bodies of dividing yeast cells.

MeSH terms

  • Cell Cycle
  • Cell Nucleus / metabolism
  • Cytoskeletal Proteins / genetics
  • Cytoskeletal Proteins / physiology*
  • Green Fluorescent Proteins
  • Luminescent Proteins / metabolism
  • Microscopy, Atomic Force
  • Microscopy, Confocal
  • Microscopy, Electron
  • Microscopy, Fluorescence
  • Microtubules / metabolism
  • Mitosis
  • Open Reading Frames
  • Protein Binding
  • Protein Structure, Tertiary
  • Recombinant Fusion Proteins / metabolism
  • Saccharomyces cerevisiae / metabolism*
  • Saccharomyces cerevisiae Proteins / genetics
  • Saccharomyces cerevisiae Proteins / physiology*
  • Spindle Apparatus*
  • Two-Hybrid System Techniques

Substances

  • Cytoskeletal Proteins
  • FIN1 protein, S cerevisiae
  • Luminescent Proteins
  • Recombinant Fusion Proteins
  • Saccharomyces cerevisiae Proteins
  • Green Fluorescent Proteins