Abstract
Pho85p is a yeast cyclin-dependent protein kinase (Cdk) that can interact with 10 cyclins (Pcls) to form multiple protein kinases. The functions of most of the Pcls, including Pc16p and Pc17p, are poorly defined. We report here that Pc16p and Pc17p are involved in the metabolism of the branched storage polysaccharide glycogen under certain conditions and deletion of PCL6 and PCL7 restores glycogen accumulation to a snf1 pcl8 pcl10 triple mutant, paradoxically activating both glycogen synthase and phosphorylase. Pho85p thus affects glycogen accumulation through multiple Cdks composed of different cyclin partners.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Cyclin-Dependent Kinases / genetics
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Cyclin-Dependent Kinases / physiology*
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Cyclins / genetics
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Cyclins / physiology*
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Enzyme Activation
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Glycogen / metabolism*
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Glycogen Phosphorylase / metabolism
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Glycogen Synthase / metabolism
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Mutation
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Protein Isoforms / genetics
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Protein Isoforms / physiology
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Saccharomyces cerevisiae / metabolism*
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Saccharomyces cerevisiae Proteins / genetics
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Saccharomyces cerevisiae Proteins / physiology*
Substances
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Cyclins
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Pcl7 protein, S cerevisiae
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Protein Isoforms
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Saccharomyces cerevisiae Proteins
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Glycogen
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Glycogen Phosphorylase
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Glycogen Synthase
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Cyclin-Dependent Kinases
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PHO85 protein, S cerevisiae