Abstract
A nuclear GTPase, Nug1p, was identified in a genetic screen for components linked to 60S ribosomal subunit export. Nug1p cosedimented with nuclear 60S preribosomes and was required for subunit export to the cytoplasm. Tagged Nug1p coprecipitated with proteins of the 60S subunit, late precursors to the 25S and 5.8S rRNAs, and at least 21 nonribosomal proteins. These included a homologous nuclear GTPase, Nug2p, the Noc2p/Noc3p heterodimer, Rix1p, and Rlp7p, each of which was implicated in 60S subunit export. Other known ribosome synthesis factors and proteins of previously unknown function, including the 559 kDa protein Ylr106p, also copurified. Eight of these proteins were copurified with nuclear pore complexes, suggesting that this complex represents the transport intermediate for 60S subunit export.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Active Transport, Cell Nucleus / physiology*
-
Amino Acid Sequence
-
Animals
-
Blotting, Northern
-
Cell Nucleus / metabolism*
-
Centrifugation, Density Gradient
-
Fungal Proteins / genetics
-
Fungal Proteins / metabolism*
-
GTP Phosphohydrolases / chemistry
-
GTP Phosphohydrolases / genetics
-
GTP Phosphohydrolases / metabolism*
-
Genes, Reporter / genetics
-
Humans
-
Molecular Sequence Data
-
Nuclear Proteins / genetics
-
Nuclear Proteins / metabolism*
-
Recombinant Fusion Proteins / genetics
-
Recombinant Fusion Proteins / metabolism
-
Ribosomal Proteins / genetics
-
Ribosomal Proteins / metabolism
-
Ribosomes / chemistry
-
Ribosomes / metabolism*
-
Saccharomyces cerevisiae / genetics
-
Saccharomyces cerevisiae / metabolism
-
Saccharomyces cerevisiae Proteins*
-
Sequence Alignment
-
Temperature
-
Transformation, Genetic
Substances
-
Fungal Proteins
-
Nuclear Proteins
-
Recombinant Fusion Proteins
-
Ribosomal Proteins
-
Saccharomyces cerevisiae Proteins
-
GTP Phosphohydrolases
-
Nug1 protein, S cerevisiae