Abstract
The FCY2 gene of Saccharomyces cerevisiae encodes a purine-cytosine permease (PCP) that mediates the active transport of purines and cytosine. A structure-function model for this PCP has been recently proposed. In this study, we developed a plasmid-based system that generated a number of affinity-mutated alleles, enabling us to define new amino acids critical for permease function.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alleles
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Carrier Proteins / genetics*
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DNA, Fungal
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Membrane Transport Proteins / genetics*
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Mutation*
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Mutation, Missense
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Nucleobase Transport Proteins
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Plasmids*
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Saccharomyces cerevisiae / enzymology*
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae Proteins*
Substances
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Carrier Proteins
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DNA, Fungal
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FCY2 protein, S cerevisiae
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Membrane Transport Proteins
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Nucleobase Transport Proteins
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Saccharomyces cerevisiae Proteins