Abstract
RNA polymerase II elongation factor ELL was recently purified from rat liver as a component of a multiprotein complex containing ELL and three ELL-associated proteins (EAPs) of approximately 45 (EAP45), approximately 30 (EAP30), and approximately 20 (EAP20) kDa (Shilatifard, A. (1998) J. Biol. Chem. 273, 11212-11217). Cloning of cDNA encoding the EAP30 protein revealed that it shares significant sequence similarity with the product of the Saccharomyces cerevisiae SNF8 gene (Schmidt, A. E., Miller, T., Schmidt, S. L., Shiekhattar, R., and Shilatifard, A. (1999) J. Biol. Chem. 274, 21981-21985), which is required for efficient derepression of glucose-repressed genes. Here we report the cloning of cDNAs encoding the EAP45 and EAP20 proteins. In addition, we identify the S. cerevisiae VPS36 and YJR102c genes as potential orthologs of EAP45 and EAP20 and show that they are previously uncharacterized SNF genes with properties very similar to SNF8.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Caenorhabditis elegans / genetics
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Cloning, Molecular
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Conserved Sequence
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DNA-Binding Proteins / chemistry
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism*
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Drosophila melanogaster / genetics
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Endosomal Sorting Complexes Required for Transport
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Fungal Proteins / chemistry
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Fungal Proteins / genetics
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Fungal Proteins / metabolism
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Gene Expression Regulation, Fungal / drug effects
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Gene Expression Regulation, Fungal / physiology*
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Glucose / pharmacology*
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Humans
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Liver / enzymology
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Mammals
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Mice
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Molecular Sequence Data
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Neoplasm Proteins*
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Peptide Elongation Factors*
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Peptide Fragments / chemistry
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Protein Subunits
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RNA Polymerase II / metabolism
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Rats
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Recombinant Proteins / metabolism
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Saccharomyces cerevisiae / drug effects
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Saccharomyces cerevisiae / genetics*
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Saccharomyces cerevisiae / metabolism
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Sequence Alignment
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Sequence Homology, Amino Acid
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Transcription Factors / chemistry
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Transcription Factors / genetics
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Transcription Factors / metabolism*
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Transcriptional Elongation Factors
Substances
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DNA-Binding Proteins
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EAP45 protein, rat
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ELL protein, human
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Endosomal Sorting Complexes Required for Transport
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Fungal Proteins
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Neoplasm Proteins
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Peptide Elongation Factors
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Peptide Fragments
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Protein Subunits
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Recombinant Proteins
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Transcription Factors
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Transcriptional Elongation Factors
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Vps25 protein, rat
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RNA Polymerase II
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Glucose