Rer1p, a retrieval receptor for endoplasmic reticulum membrane proteins, is dynamically localized to the Golgi apparatus by coatomer

J Cell Biol. 2001 Mar 5;152(5):935-44. doi: 10.1083/jcb.152.5.935.

Abstract

Rer1p, a yeast Golgi membrane protein, is required for the retrieval of a set of endoplasmic reticulum (ER) membrane proteins. We present the first evidence that Rer1p directly interacts with the transmembrane domain (TMD) of Sec12p which contains a retrieval signal. A green fluorescent protein (GFP) fusion of Rer1p rapidly cycles between the Golgi and the ER. Either a lesion of coatomer or deletion of the COOH-terminal tail of Rer1p causes its mislocalization to the vacuole. The COOH-terminal Rer1p tail interacts in vitro with a coatomer complex containing alpha and gamma subunits. These findings not only give the proof that Rer1p is a novel type of retrieval receptor recognizing the TMD in the Golgi but also indicate that coatomer actively regulates the function and localization of Rer1p.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Motifs
  • Amino Acid Sequence
  • Coat Protein Complex I / chemistry
  • Coat Protein Complex I / genetics
  • Coat Protein Complex I / metabolism*
  • Endoplasmic Reticulum / metabolism*
  • Fungal Proteins / chemistry
  • Fungal Proteins / genetics
  • Fungal Proteins / metabolism*
  • Golgi Apparatus / chemistry*
  • Golgi Apparatus / metabolism
  • Guanine Nucleotide Exchange Factors
  • Intracellular Membranes / chemistry
  • Intracellular Membranes / metabolism
  • Lysine / genetics
  • Lysine / metabolism
  • Membrane Glycoproteins / chemistry
  • Membrane Glycoproteins / metabolism*
  • Membrane Proteins / chemistry
  • Membrane Proteins / genetics
  • Membrane Proteins / metabolism*
  • Models, Biological
  • Molecular Sequence Data
  • Mutation
  • Protein Binding
  • Protein Structure, Tertiary
  • Protein Subunits
  • Protein Transport
  • Recombinant Fusion Proteins / chemistry
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Saccharomyces cerevisiae / cytology
  • Saccharomyces cerevisiae / metabolism*
  • Saccharomyces cerevisiae Proteins*
  • Sequence Alignment
  • Vacuoles / metabolism
  • Vesicular Transport Proteins

Substances

  • Coat Protein Complex I
  • Fungal Proteins
  • Guanine Nucleotide Exchange Factors
  • Membrane Glycoproteins
  • Membrane Proteins
  • Protein Subunits
  • RER1 protein, S cerevisiae
  • Recombinant Fusion Proteins
  • SEC12 protein, S cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Vesicular Transport Proteins
  • Lysine