Abstract
Isw1p and Isw2p are budding yeast homologs of the Drosophila ISWI chromatin-remodeling ATPase. Using indirect-end-label and chromatin immunoprecipitation analysis, we show both independent and cooperative Isw1p- and Isw2p-mediated positioning of short nucleosome arrays in gene-regulatory elements at a variety of transcription units in vivo. We present evidence that both yeast ISWI complexes regulate developmental responses to starvation and that for Isw2p, recruitment by different DNA-binding proteins controls meiosis and haploid invasive growth.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Adenosine Triphosphatases / chemistry
-
Adenosine Triphosphatases / metabolism*
-
Blotting, Northern
-
Chromatin / chemistry
-
Chromatin / metabolism*
-
DNA Footprinting
-
DNA, Fungal / analysis
-
Fungal Proteins / chemistry
-
Fungal Proteins / metabolism*
-
Meiosis
-
Micrococcal Nuclease
-
Nucleic Acid Hybridization
-
Saccharomyces cerevisiae / cytology*
-
Saccharomyces cerevisiae / growth & development
-
Transcription Factors / chemistry
-
Transcription Factors / metabolism*
Substances
-
Chromatin
-
DNA, Fungal
-
Fungal Proteins
-
ISWI protein
-
Transcription Factors
-
Micrococcal Nuclease
-
Adenosine Triphosphatases