In vivo chromatin remodeling by yeast ISWI homologs Isw1p and Isw2p

Genes Dev. 2001 Mar 1;15(5):619-26. doi: 10.1101/gad.190301.

Abstract

Isw1p and Isw2p are budding yeast homologs of the Drosophila ISWI chromatin-remodeling ATPase. Using indirect-end-label and chromatin immunoprecipitation analysis, we show both independent and cooperative Isw1p- and Isw2p-mediated positioning of short nucleosome arrays in gene-regulatory elements at a variety of transcription units in vivo. We present evidence that both yeast ISWI complexes regulate developmental responses to starvation and that for Isw2p, recruitment by different DNA-binding proteins controls meiosis and haploid invasive growth.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphatases / chemistry
  • Adenosine Triphosphatases / metabolism*
  • Blotting, Northern
  • Chromatin / chemistry
  • Chromatin / metabolism*
  • DNA Footprinting
  • DNA, Fungal / analysis
  • Fungal Proteins / chemistry
  • Fungal Proteins / metabolism*
  • Meiosis
  • Micrococcal Nuclease
  • Nucleic Acid Hybridization
  • Saccharomyces cerevisiae / cytology*
  • Saccharomyces cerevisiae / growth & development
  • Transcription Factors / chemistry
  • Transcription Factors / metabolism*

Substances

  • Chromatin
  • DNA, Fungal
  • Fungal Proteins
  • ISWI protein
  • Transcription Factors
  • Micrococcal Nuclease
  • Adenosine Triphosphatases