Notch inhibition of RAS signaling through MAP kinase phosphatase LIP-1 during C. elegans vulval development

T Berset; E F Hoier; G Battu; S Canevascini; A Hajnal

doi:10.1126/science.1055642

Notch inhibition of RAS signaling through MAP kinase phosphatase LIP-1 during C. elegans vulval development

Science. 2001 Feb 9;291(5506):1055-8. doi: 10.1126/science.1055642. Epub 2001 Jan 25.

Authors

T Berset¹, E F Hoier, G Battu, S Canevascini, A Hajnal

Affiliation

¹ Division of Cancer Research, Department of Pathology, University of Zürich, Schmelzbergstrasse 12, CH-8091 Zürich, Switzerland.

PMID: 11161219
DOI: 10.1126/science.1055642

Abstract

During Caenorhabditis elegans vulval development, a signal from the anchor cell stimulates the RTK/RAS/MAPK (receptor tyrosine kinase/RAS/mitogen-activated protein kinase) signaling pathway in the closest vulval precursor cell P6.p to induce the primary fate. A lateral signal from P6.p then activates the Notch signaling pathway in the neighboring cells P5.p and P7.p to prevent them from adopting the primary fate and to specify the secondary fate. The MAP kinase phosphatase LIP-1 mediates this lateral inhibition of the primary fate. LIN-12/NOTCH up-regulates lip-1 transcription in P5.p and P7.p where LIP-1 inactivates the MAP kinase to inhibit primary fate specification. LIP-1 thus links the two signaling pathways to generate a pattern.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Motifs
Amino Acid Sequence
Animals
Body Patterning
Caenorhabditis elegans / cytology
Caenorhabditis elegans / genetics
Caenorhabditis elegans / growth & development*
Caenorhabditis elegans / metabolism
Caenorhabditis elegans Proteins*
Catalytic Domain
Cell Cycle Proteins*
Female
Gene Expression Regulation
Genes, Helminth
Helminth Proteins / metabolism*
MAP Kinase Signaling System*
Membrane Proteins / metabolism*
Mitogen-Activated Protein Kinase 1
Molecular Sequence Data
Mutation
Protein Serine-Threonine Kinases / metabolism*
Protein Structure, Tertiary
Protein Tyrosine Phosphatases / chemistry
Protein Tyrosine Phosphatases / genetics*
Protein Tyrosine Phosphatases / metabolism*
Receptor Protein-Tyrosine Kinases / metabolism
Receptors, Notch
Recombinant Fusion Proteins / metabolism
Signal Transduction
Stem Cells / cytology
Stem Cells / metabolism
Up-Regulation
Vulva / cytology
Vulva / growth & development
ras Proteins / metabolism*

Substances

Caenorhabditis elegans Proteins
Cell Cycle Proteins
Helminth Proteins
Lin-12 protein, C elegans
Membrane Proteins
Receptors, Notch
Recombinant Fusion Proteins
Receptor Protein-Tyrosine Kinases
Protein Serine-Threonine Kinases
Mitogen-Activated Protein Kinase 1
mpk-1 protein, C elegans
lip-1 protein, C elegans
Protein Tyrosine Phosphatases
ras Proteins