Identification and characterization of yUAP/Sub2p, a yeast homolog of the essential human pre-mRNA splicing factor hUAP56

Genes Dev. 2001 Jan 1;15(1):30-5. doi: 10.1101/gad.851701.

Abstract

The human 56-kD U2AF(65)-associated protein (hUAP56), a member of the DExD/H box protein family of RNA-dependent ATPases, is required for the stable binding of U2 snRNP to the pre-mRNA branchpoint. Here we identify a highly conserved Saccharomyces cerevisiae homolog of hUAP56, yUAP/Sub2p. yUAP/Sub2p can be functionally substituted for by hUAP56 and, like its human counterpart, is an essential pre-mRNA splicing factor. yUAP/Sub2p is required for formation of the commitment complex, the precursor for U2 snRNP addition. In conjunction with previous studies, we conclude that at least two DExD/H box proteins, Prp5p and yUAP/Sub2p, mediate the U2 snRNP-branchpoint interaction.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenosine Triphosphatases / chemistry
  • Adenosine Triphosphatases / genetics*
  • Adenosine Triphosphatases / metabolism
  • Amino Acid Sequence
  • Cloning, Molecular
  • Conserved Sequence
  • Fungal Proteins / chemistry
  • Fungal Proteins / genetics
  • Humans
  • Molecular Sequence Data
  • Mutagenesis, Site-Directed
  • Nuclear Proteins*
  • RNA Splicing*
  • Recombinant Proteins / metabolism
  • Ribonucleoproteins / metabolism
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / growth & development
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Splicing Factor U2AF

Substances

  • Fungal Proteins
  • Nuclear Proteins
  • Recombinant Proteins
  • Ribonucleoproteins
  • Splicing Factor U2AF
  • U2AF2 protein, human
  • Adenosine Triphosphatases
  • RNA-dependent ATPase