Abstract
The human 56-kD U2AF(65)-associated protein (hUAP56), a member of the DExD/H box protein family of RNA-dependent ATPases, is required for the stable binding of U2 snRNP to the pre-mRNA branchpoint. Here we identify a highly conserved Saccharomyces cerevisiae homolog of hUAP56, yUAP/Sub2p. yUAP/Sub2p can be functionally substituted for by hUAP56 and, like its human counterpart, is an essential pre-mRNA splicing factor. yUAP/Sub2p is required for formation of the commitment complex, the precursor for U2 snRNP addition. In conjunction with previous studies, we conclude that at least two DExD/H box proteins, Prp5p and yUAP/Sub2p, mediate the U2 snRNP-branchpoint interaction.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adenosine Triphosphatases / chemistry
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Adenosine Triphosphatases / genetics*
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Adenosine Triphosphatases / metabolism
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Amino Acid Sequence
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Cloning, Molecular
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Conserved Sequence
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Fungal Proteins / chemistry
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Fungal Proteins / genetics
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Humans
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Molecular Sequence Data
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Mutagenesis, Site-Directed
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Nuclear Proteins*
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RNA Splicing*
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Recombinant Proteins / metabolism
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Ribonucleoproteins / metabolism
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Saccharomyces cerevisiae / genetics*
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Saccharomyces cerevisiae / growth & development
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Sequence Alignment
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Sequence Homology, Amino Acid
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Splicing Factor U2AF
Substances
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Fungal Proteins
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Nuclear Proteins
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Recombinant Proteins
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Ribonucleoproteins
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Splicing Factor U2AF
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U2AF2 protein, human
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Adenosine Triphosphatases
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RNA-dependent ATPase