A genetic analysis of glucocorticoid receptor signaling. Identification and characterization of ligand-effect modulators in Saccharomyces cerevisiae

Genetics. 2000 Nov;156(3):963-72. doi: 10.1093/genetics/156.3.963.

Abstract

To find novel components in the glucocorticoid signal transduction pathway, we performed a yeast genetic screen to identify ligand-effect modulators (LEMs), proteins that modulate the cellular response to hormone. We isolated several mutants that conferred increased glucocorticoid receptor (GR) activity in response to dexamethasone and analyzed two of them in detail. These studies identify two genes, LEM3 and LEM4, which correspond to YNL323w and ERG6, respectively. LEM3 is a putative transmembrane protein of unknown function, and ERG6 is a methyltransferase in the ergosterol biosynthetic pathway. Analysis of null mutants indicates that LEM3 and ERG6 act at different steps in the GR signal transduction pathway.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • ATP-Binding Cassette Transporters / genetics
  • Animals
  • Base Sequence
  • Canavanine / pharmacology
  • Cloning, Molecular
  • Crosses, Genetic
  • DNA Primers
  • Dexamethasone / pharmacology*
  • Fungal Proteins / genetics
  • Fungal Proteins / metabolism
  • Genetic Complementation Test
  • Membrane Proteins / genetics
  • Membrane Proteins / metabolism
  • Methyltransferases / genetics
  • Methyltransferases / metabolism
  • Molecular Sequence Data
  • Mutagenesis
  • Polymerase Chain Reaction
  • Receptors, Glucocorticoid / drug effects
  • Receptors, Glucocorticoid / genetics*
  • Receptors, Glucocorticoid / physiology
  • Recombinant Proteins / drug effects
  • Recombinant Proteins / metabolism
  • Saccharomyces cerevisiae / drug effects
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / physiology
  • Saccharomyces cerevisiae Proteins*
  • Signal Transduction / physiology*
  • Ultraviolet Rays

Substances

  • ATP-Binding Cassette Transporters
  • DNA Primers
  • Fungal Proteins
  • Membrane Proteins
  • PDR5 protein, S cerevisiae
  • Receptors, Glucocorticoid
  • Recombinant Proteins
  • Saccharomyces cerevisiae Proteins
  • Canavanine
  • Dexamethasone
  • Methyltransferases
  • delta 24-sterol methyltransferase