Abstract
The deletion of the TOM1 gene encoding a putative ubiquitin ligase causes a temperature sensitive cellular growth in Saccharomyces cerevisiae. The arrested cells exhibit pleiotropic defects in nuclear division, maintenance of nuclear structure and heat stress responses. We previously identified a zuo1 mutation as an extragenic suppressor of the tom1 mutant. ZUO1 encodes a DnaJ-related Hsp40. Here we show that a recessive cold sensitive mutation in PDR13 coding for an Hsp70 suppressed the tom1 mutation. The pdr13 deletion mutant was sensitive to high osmolarity, just like the zuo1 deletion mutant. A zuo1 pdr13 double deletion mutant did not show additive phenotypes. Furthermore, a tagged-Zuo1p was co-immunoprecipitated with a tagged-Pdr13p. Taken together, we propose that Pdr13p and Zuo1p are a new pair of Hsp70:Hsp40 molecular chaperones. In addition, Pdr13p co-sedimented with translating ribosomes and this association was independent of the presence of Zuo1p.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism*
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Fungal Proteins / genetics
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Fungal Proteins / metabolism*
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Gene Dosage
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HSP40 Heat-Shock Proteins
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HSP70 Heat-Shock Proteins / genetics
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HSP70 Heat-Shock Proteins / metabolism*
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Heat-Shock Proteins / genetics
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Heat-Shock Proteins / metabolism*
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Homeodomain Proteins / genetics
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Homeodomain Proteins / metabolism
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Molecular Chaperones / genetics
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Molecular Chaperones / metabolism
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Mutation
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Phenotype
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Protein Binding
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Ribosomes / metabolism
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae / metabolism*
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Saccharomyces cerevisiae Proteins*
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Schizosaccharomyces pombe Proteins*
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Suppression, Genetic
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Ubiquitin-Protein Ligases
Substances
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DNA-Binding Proteins
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Fungal Proteins
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HSP40 Heat-Shock Proteins
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HSP70 Heat-Shock Proteins
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Heat-Shock Proteins
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Homeodomain Proteins
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Molecular Chaperones
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SSB1 protein, S cerevisiae
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SSB2 protein, S pombe
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Saccharomyces cerevisiae Proteins
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Schizosaccharomyces pombe Proteins
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ZUO1 protein, S cerevisiae
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TOM1 protein, S cerevisiae
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Ubiquitin-Protein Ligases