Yeast Pdr13p and Zuo1p molecular chaperones are new functional Hsp70 and Hsp40 partners

Gene. 2000 Oct 17;257(1):131-7. doi: 10.1016/s0378-1119(00)00381-4.

Abstract

The deletion of the TOM1 gene encoding a putative ubiquitin ligase causes a temperature sensitive cellular growth in Saccharomyces cerevisiae. The arrested cells exhibit pleiotropic defects in nuclear division, maintenance of nuclear structure and heat stress responses. We previously identified a zuo1 mutation as an extragenic suppressor of the tom1 mutant. ZUO1 encodes a DnaJ-related Hsp40. Here we show that a recessive cold sensitive mutation in PDR13 coding for an Hsp70 suppressed the tom1 mutation. The pdr13 deletion mutant was sensitive to high osmolarity, just like the zuo1 deletion mutant. A zuo1 pdr13 double deletion mutant did not show additive phenotypes. Furthermore, a tagged-Zuo1p was co-immunoprecipitated with a tagged-Pdr13p. Taken together, we propose that Pdr13p and Zuo1p are a new pair of Hsp70:Hsp40 molecular chaperones. In addition, Pdr13p co-sedimented with translating ribosomes and this association was independent of the presence of Zuo1p.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism*
  • Fungal Proteins / genetics
  • Fungal Proteins / metabolism*
  • Gene Dosage
  • HSP40 Heat-Shock Proteins
  • HSP70 Heat-Shock Proteins / genetics
  • HSP70 Heat-Shock Proteins / metabolism*
  • Heat-Shock Proteins / genetics
  • Heat-Shock Proteins / metabolism*
  • Homeodomain Proteins / genetics
  • Homeodomain Proteins / metabolism
  • Molecular Chaperones / genetics
  • Molecular Chaperones / metabolism
  • Mutation
  • Phenotype
  • Protein Binding
  • Ribosomes / metabolism
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / metabolism*
  • Saccharomyces cerevisiae Proteins*
  • Schizosaccharomyces pombe Proteins*
  • Suppression, Genetic
  • Ubiquitin-Protein Ligases

Substances

  • DNA-Binding Proteins
  • Fungal Proteins
  • HSP40 Heat-Shock Proteins
  • HSP70 Heat-Shock Proteins
  • Heat-Shock Proteins
  • Homeodomain Proteins
  • Molecular Chaperones
  • SSB1 protein, S cerevisiae
  • SSB2 protein, S pombe
  • Saccharomyces cerevisiae Proteins
  • Schizosaccharomyces pombe Proteins
  • ZUO1 protein, S cerevisiae
  • TOM1 protein, S cerevisiae
  • Ubiquitin-Protein Ligases