Abstract
A new family of glycosylphosphatidylinositol-anchored beta(1-3)glucanosyltransferases (Gelp), recently identified and characterized in the filamentous fungus Aspergillus fumigatus, showed functional similarity to the Gas/Phr/Epd protein families, which are involved in yeast morphogenesis. Sequence comparisons and hydrophobic cluster analysis (HCA) showed that all the Gas/Phr/Epd/Gel proteins belong to a new family of glycosylhydrolases, family 72. We confirmed by site-directed mutagenesis and biochemical analysis that the two conserved glutamate residues (the putative catalytic residues of this family, as determined by HCA) are involved in the active site of this family of glycosylhydrolases.
MeSH terms
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Amino Acid Sequence
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Aspergillus fumigatus / enzymology*
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Binding Sites
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Blotting, Western
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Catalytic Domain*
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Conserved Sequence / genetics
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Fungal Proteins / chemistry*
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Fungal Proteins / classification
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Fungal Proteins / genetics
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Fungal Proteins / metabolism*
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Glucan Endo-1,3-beta-D-Glucosidase / chemistry*
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Glucan Endo-1,3-beta-D-Glucosidase / classification
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Glucan Endo-1,3-beta-D-Glucosidase / genetics
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Glucan Endo-1,3-beta-D-Glucosidase / metabolism*
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Glutamic Acid / genetics
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Glutamic Acid / metabolism
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Glycosylphosphatidylinositols / metabolism
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Molecular Sequence Data
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Multigene Family / genetics
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Mutation / genetics
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Protein Structure, Secondary
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Recombinant Proteins / chemistry
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Recombinant Proteins / classification
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Recombinant Proteins / genetics
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Recombinant Proteins / metabolism
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Sequence Alignment
Substances
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Fungal Proteins
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Glycosylphosphatidylinositols
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Recombinant Proteins
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Glutamic Acid
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1,3-beta-glucanosyltransferase
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Glucan Endo-1,3-beta-D-Glucosidase
Associated data
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GENBANK/AF072700
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GENBANK/AF208039
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GENBANK/AF208040