Abstract
Selective movement of proteins between the nucleus and the cytoplasm is a regulatory mechanism exploited extensively by the eukaryotic cell. We have identified the evolutionarily conserved Sac3 protein, which was implicated previously in the regulation of mitosis [Bauer, A. & Kölling, R. (1996) J. Cell Sci. 109, 1575-1583] as a novel mediator of nuclear protein export. We show that Sac3p is localized to the nuclear pore, where it interacts with nucleoporins. Loss of SAC3 function results in a block in nuclear export of a nuclear export signal-containing reporter protein. Our results also demonstrate that SAC3 interacts genetically with the nuclear protein export factors Crm1p/Xpo1p and Yrb2p. Taken together, these data indicate a link between nuclear protein export and transition through the cell cycle.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Biological Transport
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Calcium-Binding Proteins*
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Cell Cycle*
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Fluorescent Antibody Technique, Indirect
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Fungal Proteins / metabolism
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Gene Deletion
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Green Fluorescent Proteins
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Luminescent Proteins / genetics
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Molecular Sequence Data
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Nuclear Pore Complex Proteins
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Nuclear Proteins / genetics
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Nuclear Proteins / metabolism*
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Nucleocytoplasmic Transport Proteins
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Porins
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Protein Binding
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
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Saccharomyces cerevisiae Proteins*
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Sequence Homology, Amino Acid
Substances
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Calcium-Binding Proteins
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Fungal Proteins
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Luminescent Proteins
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NSP1 protein, S cerevisiae
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Nuclear Pore Complex Proteins
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Nuclear Proteins
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Nucleocytoplasmic Transport Proteins
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Porins
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Recombinant Fusion Proteins
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SAC3 protein, S cerevisiae
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Saccharomyces cerevisiae Proteins
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Green Fluorescent Proteins