Abstract
The proper folding of tubulins and their incorporation into microtubules consist of a series of reactions, in which evolutionarily conserved proteins, cofactors A to E, play a vital role. We have cloned a fission yeast gene (alp41(+)) which encodes a highly conserved small GTP-binding protein homologous to budding yeast CIN4 and human ARF-like Arl2. alp41(+) is essential, disruption of which results in microtubule dysfunction and growth polarity defects. Genetic analysis indicates that Alp41 plays a crucial role in the cofactor-dependent pathway, in which it functions upstream of the cofactor D homologue Alp1(D) and possibly in concert with Alp21(E).
MeSH terms
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Cell Division / genetics
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Cloning, Molecular
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Conserved Sequence
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Fungal Proteins / genetics
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Fungal Proteins / metabolism
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GTP-Binding Proteins / genetics*
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GTP-Binding Proteins / metabolism*
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Gene Deletion
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Humans
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Microtubule-Associated Proteins / genetics
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Microtubule-Associated Proteins / metabolism
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Microtubules / metabolism*
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Molecular Sequence Data
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Phylogeny
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Protein Folding
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae / metabolism
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Saccharomyces cerevisiae Proteins*
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Schizosaccharomyces / genetics
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Schizosaccharomyces / metabolism*
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Schizosaccharomyces pombe Proteins*
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Sequence Alignment
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Sequence Homology, Amino Acid
Substances
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Alp1 protein, S pombe
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Alp41 protein, S pombe
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CIN1 protein, S cerevisiae
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Fungal Proteins
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Microtubule-Associated Proteins
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Saccharomyces cerevisiae Proteins
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Schizosaccharomyces pombe Proteins
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GTP-Binding Proteins