Abstract
The Saccharomyces cerevisiae YDR033w gene product is homologous to Hsp30p and Yro2p, both of which are induced during heat shock. To investigate the subcellular localization of the YDR033w gene product, hemagglutinin (HA) epitope-tagged protein was expressed, detected on immunoblots, and localized by immunofluorescence to cell membranes, primarily the plasma membrane. A punctuate immunofluorescence pattern was observed within cell buds. The nuclear envelope, but not the vacuole or mitochondrial membranes, were also immunostained. We refer to YDR033w as MRH1 to denote that it encodes a membrane protein related to Hsp30p.
MeSH terms
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Amino Acid Sequence
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HSP30 Heat-Shock Proteins
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Heat-Shock Proteins / chemistry
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Heat-Shock Proteins / genetics*
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Hemagglutinins / genetics
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Hot Temperature
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Membrane Proteins / chemistry
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Membrane Proteins / genetics*
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Molecular Sequence Data
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Recombinant Proteins / biosynthesis
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Recombinant Proteins / chemistry
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Saccharomyces cerevisiae / genetics*
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Saccharomyces cerevisiae / metabolism*
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Saccharomyces cerevisiae Proteins*
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Sequence Alignment
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Sequence Homology, Amino Acid
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Subcellular Fractions / metabolism
Substances
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HSP30 Heat-Shock Proteins
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HSP30 protein, S cerevisiae
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Heat-Shock Proteins
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Hemagglutinins
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MRH1 protein, S cerevisiae
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Membrane Proteins
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Recombinant Proteins
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Saccharomyces cerevisiae Proteins