Nuclear tRNA aminoacylation and its role in nuclear export of endogenous tRNAs in Saccharomyces cerevisiae

S Sarkar; A K Azad; A K Hopper

doi:10.1073/pnas.96.25.14366

Nuclear tRNA aminoacylation and its role in nuclear export of endogenous tRNAs in Saccharomyces cerevisiae

Proc Natl Acad Sci U S A. 1999 Dec 7;96(25):14366-71. doi: 10.1073/pnas.96.25.14366.

Authors

S Sarkar¹, A K Azad, A K Hopper

Affiliation

¹ Department of Biochemistry, Pennsylvania State University, College of Medicine, Hershey, PA, 17033, USA.

Abstract

Nuclear tRNA aminoacylation was proposed to provide a proofreading step in Xenopus oocytes, ensuring nuclear export of functional tRNAs [Lund, E. & Dahlberg, J. E. (1998) Science 282, 2082-2085]. Herein, it is documented that tRNA aminoacylation also occurs in yeast nuclei and is important for tRNA export. We propose that tRNA aminoacylation functions in one of at least two parallel paths of tRNA export in yeast. Alteration of one aminoacyl-tRNA synthetase affects export of only cognate tRNA, whereas alterations of two other aminoacyl-tRNA synthetases affect export of both cognate and noncognate tRNAs. Saturation of tRNA export pathway is a possible explanation of this phenomenon.

Publication types

Research Support, U.S. Gov't, P.H.S.

MeSH terms

Amino Acyl-tRNA Synthetases / physiology*
Biological Transport
Cell Nucleus / metabolism*
RNA, Transfer / metabolism*
Saccharomyces cerevisiae / metabolism*

Substances

RNA, Transfer
Amino Acyl-tRNA Synthetases