Abstract
DHS-1-P phosphatases cloned from yeast represent novel lipid phosphatases, which were not thought to exist in yeast. Identification and characterization of YSR2 and YSR3 have demonstrated that the DHS-1-P phosphatase is an important mediator in the biosynthesis of sphingolipids and in the maintenance of the balance of signaling lipid molecules ceramide, sphingosine, and sphingosine-1-P. Methods introduced here for purification, activity assay, in vivo labeling, and cellular localization using GFP tagging are expected to facilitate our understanding of this enzyme.
MeSH terms
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Cell Compartmentation
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Cloning, Molecular
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Genes, Fungal
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Heat-Shock Response
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Mutagenesis
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Oligopeptides
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Palmitic Acid / metabolism
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Peptides
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Phosphoric Monoester Hydrolases / genetics
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Phosphoric Monoester Hydrolases / metabolism*
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Phosphorus Radioisotopes
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Saccharomyces cerevisiae / enzymology*
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Saccharomyces cerevisiae Proteins*
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Serine / metabolism
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Sphingolipids / metabolism
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Sphingosine / analogs & derivatives*
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Sphingosine / metabolism
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Tritium
Substances
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Oligopeptides
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Peptides
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Phosphorus Radioisotopes
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Saccharomyces cerevisiae Proteins
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Sphingolipids
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Tritium
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dihydrosphingosine 1-phosphate
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Palmitic Acid
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Serine
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FLAG peptide
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LCB3 protein, S cerevisiae
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YSR3 protein, S cerevisiae
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Phosphoric Monoester Hydrolases
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Sphingosine