Abstract
In metazoans, certain calmodulin-related calcium-binding proteins (recoverins, neurocalcins and frequenins) are found at highest levels in excitable cells, but their physiological roles are largely uncharacterized. Here we show that Saccharomyces cerevisiae contains a frequenin homologue, Frq1, and that its target is Pik1, a phosphatidylinositol-4-OH kinase. Frq1 binds to a conserved sequence motif in Pik1 outside Pik1's catalytic domain and stimulates its activity in vitro. N-myristoylated Frq1 may also assist in Pik1 localization.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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1-Phosphatidylinositol 4-Kinase / genetics
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1-Phosphatidylinositol 4-Kinase / metabolism*
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Amino Acid Sequence
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Animals
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Binding Sites / genetics
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Calcium-Binding Proteins / genetics
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Calcium-Binding Proteins / metabolism*
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Conserved Sequence
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Enzyme Activation
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Fungal Proteins / genetics
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Fungal Proteins / metabolism*
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Genes, Fungal
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Molecular Sequence Data
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Nerve Tissue Proteins / genetics
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Nerve Tissue Proteins / metabolism*
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae / metabolism*
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Saccharomyces cerevisiae Proteins*
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Sequence Homology, Amino Acid
Substances
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Calcium-Binding Proteins
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FRQ1 protein, S cerevisiae
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Fungal Proteins
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Nerve Tissue Proteins
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Saccharomyces cerevisiae Proteins
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1-Phosphatidylinositol 4-Kinase
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PIK1 protein, S cerevisiae