Abstract
We have analyzed Ca2+ transport activity in defined subcellular fractions of an isogenic set of wild-type and mutant yeast. The results, together with measurements of polypeptide expression levels and promoter::reporter gene activity, show that the Golgi Ca2+-ATPase, Pmr1, is the major Ca2+ pump under normal growth conditions. In the absence of Pmr1, we show a massive, calcineurin-dependent compensatory induction of the vacuolar Ca2+-ATPase, Pmc1. In addition, H+/Ca2+ exchange activity, that may be distinct from the vacuolar exchanger Vcx1, is also increased.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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ATP-Binding Cassette Transporters / genetics
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ATP-Binding Cassette Transporters / metabolism*
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Calcium / metabolism
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Calcium-Transporting ATPases / metabolism*
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Golgi Apparatus / metabolism
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Hydrogen / metabolism
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Ion Transport
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Molecular Chaperones
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Saccharomyces cerevisiae / metabolism*
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Saccharomyces cerevisiae / ultrastructure
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Saccharomyces cerevisiae Proteins*
Substances
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ATP-Binding Cassette Transporters
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Molecular Chaperones
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SSC1 protein, S cerevisiae
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Saccharomyces cerevisiae Proteins
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Hydrogen
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Calcium-Transporting ATPases
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Calcium