Abstract
Autophagy is an intracellular bulk degradation system that is ubiquitous for eukaryotic cells. In this process, cytoplasmic components are enclosed in autophagosomes and delivered to lysosomes/vacuoles. We recently found that a protein conjugation system, in which Apg12p is covalently attached to Apg5p, is indispensable for autophagy in yeast. Here, we describe a novel coiled-coil protein, Apg16p, essential for autophagy. Apg16p interacts with Apg12p-conjugated Apg5p and less preferentially with unconjugated Apg5p. Moreover, the coiled-coil domain of Apg16p mediates self-multimerization that leads to cross-linking of Apg5p molecules and formation of a stable protein complex. Apg16p is not essential for the Apg12p-Apg5p conjugation reaction. These results suggest that the Apg12p-Apg5p conjugate requires Apg16p to accomplish its role in the autophagy pathway, and Apg16p is a key molecule as a linker to form the Apg12p-Apg5p-Apg16p multimer.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Autophagy*
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Autophagy-Related Protein 5
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Autophagy-Related Protein 7
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Autophagy-Related Proteins
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Carrier Proteins / chemistry
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Carrier Proteins / genetics
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Carrier Proteins / metabolism*
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Cell Membrane / metabolism
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Cloning, Molecular
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Fungal Proteins / genetics
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Fungal Proteins / metabolism
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Gene Expression
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Genes, Essential / genetics
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Genes, Essential / physiology
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Genes, Fungal / genetics
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Genes, Fungal / physiology
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Membrane Proteins / chemistry
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Membrane Proteins / genetics
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Membrane Proteins / metabolism*
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Molecular Sequence Data
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Precipitin Tests
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Protein Binding
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Proteins / genetics
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Proteins / metabolism*
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Saccharomyces cerevisiae / cytology
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae / metabolism
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Saccharomyces cerevisiae / physiology*
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Saccharomyces cerevisiae Proteins*
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Sequence Deletion
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Transcription Factors*
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Ubiquitin-Protein Ligases
Substances
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ATG16 protein, S cerevisiae
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ATG5 protein, S cerevisiae
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ATG7 protein, S cerevisiae
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Autophagy-Related Protein 5
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Autophagy-Related Proteins
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Carrier Proteins
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Fungal Proteins
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Membrane Proteins
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Proteins
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Saccharomyces cerevisiae Proteins
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Transcription Factors
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Ubiquitin-Protein Ligases
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Autophagy-Related Protein 7