Abstract
An engineered mutant of Saccharomyces cerevisiae affected in biotin biosynthesis has been isolated. This mutant allowed the characterization of a bio cluster (BIO3-4-5). We demonstrate that BIO3 (YNR058w) and BIO4 (YNR057c) encode, respectively, a 7, 8-diaminopelargonic acid aminotransferase and a dethiobiotin synthase, involved in the biotin biosynthesis pathway. A novel gene, BIO5 (YNR056c), is present immediately downstream from BIO4. This gene encodes Bio5p, a protein with 11 putative transmembrane regions. Uptake experiments performed with labeled 7-keto 8-aminopelargonic acid indicate that Bio5p is responsible for transport into the cell of 7-keto 8-aminopelargonic acid.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acids / metabolism*
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Amino Acids, Diamino / metabolism
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Biological Transport
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Biotin / biosynthesis*
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Biotin / genetics
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Carbon-Nitrogen Ligases / genetics
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Carbon-Nitrogen Ligases / metabolism
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DNA Transposable Elements
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Genes, Fungal
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Membrane Proteins / genetics
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Membrane Proteins / metabolism
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Molecular Sequence Data
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Multigene Family*
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Mutation
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Phenotype
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Saccharomyces cerevisiae / enzymology
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Saccharomyces cerevisiae / genetics*
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Saccharomyces cerevisiae / growth & development
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Saccharomyces cerevisiae Proteins / genetics
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Saccharomyces cerevisiae Proteins / metabolism
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Sequence Analysis, DNA
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Transaminases / genetics
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Transaminases / metabolism
Substances
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Amino Acids
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Amino Acids, Diamino
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BIO5 protein, S cerevisiae
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DNA Transposable Elements
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Membrane Proteins
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Saccharomyces cerevisiae Proteins
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7,8-diaminopelargonic acid
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7-keto-8-aminopelargonic acid
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Biotin
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7,8-diaminopelargonic acid aminotransferase
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Transaminases
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Carbon-Nitrogen Ligases
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dethiobiotin synthetase
Associated data
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GENBANK/U47112
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GENBANK/U53467
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GENBANK/U90440