Abstract
Background:
The NPS1/STH1 gene of Saccharomyces cerevisiae is essential for mitotic growth, especially for the progression through the G2/M phase. It encodes a major component of the chromatin-remodelling complex, RSC, of unknown function. We attempted to address the function of NPS1 in meiosis.
Results:
The homozygote of the temperature sensitive nps1 mutant, nps1-105, showed reduced and delayed levels of sporulation, accompanied with a notable decrease and delay of the expression of several early meiotic genes (IME2, SPO11 and SPO13). Deletion analysis of the IME2 promoter revealed that the defect in the gene expression occurred through the URS1 site. The sporulation defect of nps1-105 was alleviated by the over-expression of either IME1 or IME2. However, over-expression of IME1 did not permit the full expression of IME2, SPO11 and SPO13 in nps1-105. In addition, the expression of NPS1 itself increased transiently upon initiation of meiosis, before the appearance of the IME2 message but after that of IME1. The impaired increase in NPS1 transcription led to inefficient sporulation.
Conclusion:
The results suggest that Nps1p/RSC is required for the activation of gene expression at the initiation of meiosis.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Cell Cycle Proteins / genetics*
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Cell Cycle Proteins / metabolism
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Chromatin / genetics*
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DNA-Binding Proteins / genetics*
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DNA-Binding Proteins / metabolism
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Fungal Proteins / genetics*
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Fungal Proteins / metabolism
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Gene Expression Regulation, Fungal*
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Intracellular Signaling Peptides and Proteins
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Meiosis / genetics*
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Mutation
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Myo-Inositol-1-Phosphate Synthase / genetics
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Nuclear Proteins / genetics
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Protein Kinases / genetics
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Protein Serine-Threonine Kinases
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Recombinant Proteins / genetics
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Recombinant Proteins / metabolism
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Regulatory Sequences, Nucleic Acid
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Saccharomyces cerevisiae / genetics*
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Saccharomyces cerevisiae Proteins*
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Spores, Fungal / genetics
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Temperature
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Transcription Factors / genetics*
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Transcription Factors / metabolism
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beta-Galactosidase / genetics
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beta-Galactosidase / metabolism
Substances
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Cell Cycle Proteins
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Chromatin
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DNA-Binding Proteins
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Fungal Proteins
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IME1 protein, S cerevisiae
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Intracellular Signaling Peptides and Proteins
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Nuclear Proteins
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Recombinant Proteins
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SPO13 protein, S cerevisiae
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Saccharomyces cerevisiae Proteins
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Transcription Factors
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Protein Kinases
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IME2 protein, S cerevisiae
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Protein Serine-Threonine Kinases
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beta-Galactosidase
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STH1 protein, S cerevisiae
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Myo-Inositol-1-Phosphate Synthase