Abstract
The Saccharomyces cerevisiae STT3 (ScSTT3) gene encodes a protein which is involved in protein glycosylation via the regulation of oligosaccharyltransferase activity. We have cloned and isolated the Schizosaccharomyces pombe STT3 homologous gene (Spstt3+). The Spstt3+ gene encodes a protein consisting of 749 amino acid residues which has significant homology with ScStt3p and the mouse Stt3p-homologue Itm1p. Disruption of the Spstt3+ gene shows that this gene is essential for growth. Like Itm1, Spstt3+ partially suppressed the temperature sensitivity of the stt3-1 mutation of S. cerevisiae, indicating that Spstt3+ is a functional and structural homologue of the ScSTT3 gene.
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Cloning, Molecular
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Conserved Sequence / genetics
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Fungal Proteins / chemistry
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Fungal Proteins / genetics
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Fungal Proteins / metabolism
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Gene Deletion
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Gene Expression Regulation, Fungal*
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Genes, Essential / genetics
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Genetic Complementation Test
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Glycosylation
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Hexosyltransferases*
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Membrane Proteins / chemistry
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Membrane Proteins / genetics*
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Membrane Proteins / metabolism
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Mice
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Molecular Sequence Data
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Mutagenesis, Insertional
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Polymerase Chain Reaction
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Saccharomyces cerevisiae / enzymology
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Saccharomyces cerevisiae / genetics*
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Saccharomyces cerevisiae Proteins*
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Schizosaccharomyces / enzymology
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Schizosaccharomyces / genetics*
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Schizosaccharomyces / growth & development
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Sequence Alignment
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Temperature
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Transferases / genetics
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Transferases / metabolism*
Substances
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Fungal Proteins
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Membrane Proteins
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Saccharomyces cerevisiae Proteins
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Transferases
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Hexosyltransferases
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STT3 protein, S cerevisiae
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dolichyl-diphosphooligosaccharide - protein glycotransferase