Abstract
A new Saccharomyces cerevisiae gene, XPT1, was isolated as a multicopy suppressor of a hypoxanthine phosphoribosyl transferase (HPRT) defect. Disruption of XPT1 affects xanthine utilization in vivo and results in a severe reduction of xanthine phosphoribosyl transferase (XPRT) activity while HPRT is unaffected. We conclude that XPT1 encodes XPRT in yeast.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Genes, Fungal*
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Hypoxanthine Phosphoribosyltransferase / genetics
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Pentosyltransferases / genetics*
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Purines / metabolism
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Saccharomyces cerevisiae / enzymology
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Saccharomyces cerevisiae / genetics*
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Suppression, Genetic
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Xanthine / metabolism*
Substances
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Purines
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Xanthine
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Pentosyltransferases
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xanthine phosphoribosyltransferase
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Hypoxanthine Phosphoribosyltransferase