This is a companion web site for the manuscript "Localization of Proteins that are Coordinately Expressed with Cln2 during the Cell Cycle" by Bryan A. Sundin, Chun-Hwei Chiu, Michael Riffle, Trisha N. Davis, and Eric G. D. Muller^* at the Department of Biochemistry, University of Washington, Seattle, WA 98195. We present the data for all the localizations that gave a detectable signal (Table1).

Abstract:

The localization of proteins can give important clues into their function and help sort data from large scale proteomic screens. A group of 45 proteins was tagged with the GFP variant YFP. These proteins were previously found to be encoded by genes that display strong cell cycle dependent expression peaking in G1. Most of these proteins localize to either the nucleus or to sites of cell growth. We are able to assign new cellular component GO terms to ASF2, TOS4, RTT109, YBR070C, YKR090W, YOL007C, YOL019W, and YPR174C. We also have novel localization data for 9 other proteins. Noteworthy localizations were found for Rfa1, a member of the DNA replication A complex, and Pri2, a subunit of the alpha DNA polymerase:primase complex. In addition to its nuclear localization Rfa1 assembled into cytoplasmic foci adjacent to the nucleus in cells at G1/S in the cell cycle. Pri2 took on a beaded appearance at the G1/S transition and later in the cell cycle was enriched in the nuclear envelope.